Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4211722..4212219 Replicon chromosome
Accession NZ_CP111008
Organism Escherichia coli strain J53-p130

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ORI87_RS20635 Protein ID WP_001173343.1
Coordinates 4211722..4211895 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ORI87_RS20640 Protein ID WP_000398619.1
Coordinates 4211923..4212219 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI87_RS20625 (4208392) 4208392..4209849 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
ORI87_RS20630 (4210086) 4210086..4211603 + 1518 WP_001295074.1 lysine--tRNA ligase -
ORI87_RS20635 (4211722) 4211722..4211895 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ORI87_RS20640 (4211923) 4211923..4212219 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ORI87_RS20645 (4212446) 4212446..4212718 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
ORI87_RS20650 (4212730) 4212730..4212960 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
ORI87_RS20655 (4213141) 4213141..4214772 + 1632 WP_001216477.1 sensor histidine kinase -
ORI87_RS20660 (4214769) 4214769..4215488 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T264795 WP_001173343.1 NZ_CP111008:c4211895-4211722 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT264795 WP_000398619.1 NZ_CP111008:c4212219-4211923 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References