Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4212129..4212626 Replicon chromosome
Accession NZ_CP110999
Organism Escherichia coli strain J53-p133

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ORI88_RS20640 Protein ID WP_001173343.1
Coordinates 4212129..4212302 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ORI88_RS20645 Protein ID WP_000398619.1
Coordinates 4212330..4212626 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI88_RS20630 (4208799) 4208799..4210256 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
ORI88_RS20635 (4210493) 4210493..4212010 + 1518 WP_001295074.1 lysine--tRNA ligase -
ORI88_RS20640 (4212129) 4212129..4212302 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ORI88_RS20645 (4212330) 4212330..4212626 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ORI88_RS20650 (4212853) 4212853..4213125 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
ORI88_RS20655 (4213137) 4213137..4213367 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
ORI88_RS20660 (4213548) 4213548..4215179 + 1632 WP_001216477.1 sensor histidine kinase -
ORI88_RS20665 (4215176) 4215176..4215895 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T264738 WP_001173343.1 NZ_CP110999:c4212302-4212129 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT264738 WP_000398619.1 NZ_CP110999:c4212626-4212330 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References