Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 84083..85706 Replicon plasmid pYUXYEH3314-1
Accession NZ_CP110989
Organism Escherichia coli strain XYEH3314

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag ORI32_RS23855 Protein ID WP_000088046.1
Coordinates 84381..85706 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag ORI32_RS23850 Protein ID WP_024136327.1
Coordinates 84083..84376 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI32_RS23820 (ORI32_23805) 79955..81196 + 1242 WP_001053338.1 VWA domain-containing protein -
ORI32_RS23825 (ORI32_23810) 81287..81418 + 132 WP_000374059.1 hypothetical protein -
ORI32_RS23830 (ORI32_23815) 81759..82040 - 282 WP_000077926.1 hypothetical protein -
ORI32_RS23835 (ORI32_23820) 82090..82281 - 192 WP_000398480.1 hypothetical protein -
ORI32_RS23840 (ORI32_23825) 82373..82714 - 342 WP_001151575.1 hypothetical protein -
ORI32_RS23845 (ORI32_23830) 83087..83479 + 393 WP_000341066.1 hypothetical protein -
ORI32_RS23850 (ORI32_23835) 84083..84376 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
ORI32_RS23855 (ORI32_23840) 84381..85706 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
ORI32_RS23860 (ORI32_23845) 85767..85973 + 207 WP_000134171.1 hypothetical protein -
ORI32_RS23865 (ORI32_23850) 86075..86485 + 411 WP_000985911.1 hypothetical protein -
ORI32_RS23870 (ORI32_23855) 86498..87313 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
ORI32_RS23875 (ORI32_23860) 87597..87779 + 183 Protein_84 IS1-like element transposase -
ORI32_RS23880 (ORI32_23865) 87832..88536 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
ORI32_RS23885 (ORI32_23870) 88726..89586 - 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
ORI32_RS23890 (ORI32_23875) 89769..90326 - 558 WP_001235713.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / mph(A) / aac(3)-IId / sul1 / qacE / qnrB6 - 1..214754 214754


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T264682 WP_000088046.1 NZ_CP110989:84381-85706 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT264682 WP_024136327.1 NZ_CP110989:84083-84376 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References