Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4266429..4267024 Replicon chromosome
Accession NZ_CP110988
Organism Escherichia coli strain XYEH3314

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag ORI32_RS20750 Protein ID WP_000239579.1
Coordinates 4266429..4266779 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag ORI32_RS20755 Protein ID WP_001223208.1
Coordinates 4266773..4267024 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI32_RS20730 (4261878) 4261878..4262900 - 1023 WP_001353963.1 ABC transporter permease -
ORI32_RS20735 (4262914) 4262914..4264416 - 1503 WP_000210557.1 sugar ABC transporter ATP-binding protein -
ORI32_RS20740 (4264556) 4264556..4265512 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
ORI32_RS20745 (4265822) 4265822..4266352 + 531 WP_000055072.1 inorganic diphosphatase -
ORI32_RS20750 (4266429) 4266429..4266779 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
ORI32_RS20755 (4266773) 4266773..4267024 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
ORI32_RS20760 (4267236) 4267236..4267577 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
ORI32_RS20765 (4267580) 4267580..4271359 - 3780 WP_053276653.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T264680 WP_000239579.1 NZ_CP110988:c4266779-4266429 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T264680 NZ_CP110988:c4266779-4266429 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT264680 WP_001223208.1 NZ_CP110988:c4267024-4266773 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT264680 NZ_CP110988:c4267024-4266773 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References