Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 5448..6246 Replicon plasmid pYUXYEH3934-1
Accession NZ_CP110979
Organism Escherichia coli strain XYEH3934

Toxin (Protein)


Gene name ataT Uniprot ID -
Locus tag ORI33_RS22590 Protein ID WP_032187665.1
Coordinates 5448..5969 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag ORI33_RS22595 Protein ID WP_001711191.1
Coordinates 5977..6246 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI33_RS22555 (ORI33_22550) 1527..2231 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
ORI33_RS22560 (ORI33_22555) 2281..2373 + 93 Protein_2 tail fiber protein -
ORI33_RS22565 (ORI33_22560) 2370..3272 + 903 WP_053291286.1 macro domain-containing protein -
ORI33_RS22570 (ORI33_22565) 3281..3862 + 582 WP_063122786.1 tail fiber assembly protein -
ORI33_RS22575 (ORI33_22570) 3996..4319 + 324 WP_001711185.1 hypothetical protein -
ORI33_RS22580 (ORI33_22575) 4333..5025 + 693 WP_024172707.1 membrane protein -
ORI33_RS22585 (ORI33_22580) 5028..5279 + 252 WP_023135660.1 hypothetical protein -
ORI33_RS22590 (ORI33_22585) 5448..5969 - 522 WP_032187665.1 GNAT family N-acetyltransferase Toxin
ORI33_RS22595 (ORI33_22590) 5977..6246 - 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
ORI33_RS22600 (ORI33_22595) 6566..7231 + 666 WP_023135658.1 AAA family ATPase -
ORI33_RS22605 (ORI33_22600) 7237..7590 + 354 WP_161953766.1 hypothetical protein -
ORI33_RS22610 (ORI33_22605) 7643..8410 - 768 WP_001711193.1 hypothetical protein -
ORI33_RS22615 (ORI33_22610) 8693..9418 + 726 WP_032187666.1 hypothetical protein -
ORI33_RS22620 (ORI33_22615) 9479..10819 + 1341 WP_097296309.1 DnaB-like helicase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid fosA3 / blaCTX-M-14 / dfrA14 / qnrS1 / tet(A) / mef(B) / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 - 1..188533 188533
- flank IS/Tn - - 1527..2231 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(48-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19345.11 Da        Isoelectric Point: 7.7881

>T264660 WP_032187665.1 NZ_CP110979:c5969-5448 [Escherichia coli]
VSNTTIEIFSGENDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT264660 WP_001711191.1 NZ_CP110979:c6246-5977 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References