Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4034936..4035512 Replicon chromosome
Accession NZ_CP110978
Organism Escherichia coli strain XYEH3934

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag ORI33_RS19370 Protein ID WP_001299718.1
Coordinates 4035225..4035512 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7U9DAW4
Locus tag ORI33_RS19365 Protein ID WP_000063150.1
Coordinates 4034936..4035238 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI33_RS19345 (4030627) 4030627..4031139 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
ORI33_RS19350 (4031532) 4031532..4033682 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
ORI33_RS19355 (4033732) 4033732..4033935 + 204 WP_000467859.1 YbdD/YjiX family protein -
ORI33_RS19360 (4033946) 4033946..4034902 + 957 WP_001297640.1 GTPase -
ORI33_RS19365 (4034936) 4034936..4035238 - 303 WP_000063150.1 BrnA antitoxin family protein Antitoxin
ORI33_RS19370 (4035225) 4035225..4035512 - 288 WP_001299718.1 BrnT family toxin Toxin
ORI33_RS19375 (4035709) 4035709..4036875 + 1167 WP_096310305.1 restriction endonuclease -
ORI33_RS19380 (4036939) 4036939..4038231 + 1293 Protein_3788 class I SAM-dependent DNA methyltransferase -
ORI33_RS19385 (4038297) 4038297..4039459 + 1163 WP_154166471.1 IS3-like element IS3 family transposase -
ORI33_RS19390 (4039490) 4039490..4040326 - 837 Protein_3790 BREX system Lon protease-like protein BrxL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T264657 WP_001299718.1 NZ_CP110978:c4035512-4035225 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11532.03 Da        Isoelectric Point: 9.4646

>AT264657 WP_000063150.1 NZ_CP110978:c4035238-4034936 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAW4

References