Detailed information of TA system TA026450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 318578..319149
• Replicon: chromosome
• Accession: NC_017312
• Organism: Enterococcus faecalis 62

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EF62_RS01550
• Protein ID: WP_002354774.1
• Coordinates: 318578..318919 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: EF62_RS01555
• Protein ID: WP_002354773.1
• Coordinates: 318919..319149 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EF62_RS01540 (314593)	314593..318207	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
EF62_RS01550 (318578)	318578..318919	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EF62_RS01555 (318919)	318919..319149	-	231	WP_002354773.1	hypothetical protein	Antitoxin
EF62_RS01560 (319325)	319325..319540	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
EF62_RS01565 (319679)	319679..320671	+	993	WP_002354765.1	biotin--[acetyl-CoA-carboxylase] ligase	-
EF62_RS01570 (320935)	320935..321564	-	630	WP_002354763.1	lytic polysaccharide monooxygenase	-
EF62_RS01580 (322257)	322257..323873	+	1617	WP_002381036.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T26450 WP_002354774.1 NC_017312:c318919-318578 [Enterococcus faecalis 62]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T26450 NC_017312:c318919-318578 [Enterococcus faecalis 62]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT26450 WP_002354773.1 NC_017312:c319149-318919 [Enterococcus faecalis 62]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT26450 NC_017312:c319149-318919 [Enterococcus faecalis 62]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812