Detailed information of TA system TA264480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 77..551
• Replicon: plasmid pCF1807-6
• Accession: NZ_CP110895
• Organism: Citrobacter freundii strain CF1807

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S1DZ70
• Locus tag: OQW59_RS28100
• Protein ID: WP_000262326.1
• Coordinates: 77..343 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: OQW59_RS28105
• Protein ID: WP_000879771.1
• Coordinates: 327..551 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQW59_RS28100 (OQW59_28100)	77..343	-	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OQW59_RS28105 (OQW59_28105)	327..551	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
OQW59_RS28110 (OQW59_28110)	1097..1285	+	189	WP_000165998.1	Rop family plasmid primer RNA-binding protein	-
OQW59_RS28115 (OQW59_28115)	1333..1533	-	201	WP_024148903.1	hypothetical protein	-
OQW59_RS28120 (OQW59_28120)	2704..3135	+	432	WP_053274236.1	DUF6530 family protein	-
OQW59_RS28125 (OQW59_28125)	3227..3733	+	507	WP_265970816.1	hypothetical protein	-
OQW59_RS28130 (OQW59_28130)	3730..4335	-	606	WP_265969940.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..13336	13336
	-	inside	IScluster/Tn	-	-	3730..13221	9491

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.00 Da
• Isoelectric Point: 9.5211

>T264480 WP_000262326.1 NZ_CP110895:c343-77 [Citrobacter freundii]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T264480 NZ_CP110895:c343-77 [Citrobacter freundii]
TTGGTCTGGACGATTAACTATTCCGACCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGGATTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCATTCTGTGTGAGATCCGGGATGACGAGCTGGTTATCCTTGCCGCCACCATT
GGCCATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT264480 WP_000879771.1 NZ_CP110895:c551-327 [Citrobacter freundii]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT264480 NZ_CP110895:c551-327 [Citrobacter freundii]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
CTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAAGATTATTATCTTTCAGCAGAAACTGCCGCACGCGTTC
GCCGTGGTGATGAAGCCGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: S1DZ70

Antitoxin

• AlphaFold DB: S1EJF5