Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 6119..6855 Replicon plasmid pMCR10_SCLZS19
Accession NZ_CP110881
Organism Enterobacter kobei strain SCLZS19

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag OQE50_RS26960 Protein ID WP_003026803.1
Coordinates 6373..6855 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag OQE50_RS26955 Protein ID WP_003026799.1
Coordinates 6119..6385 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQE50_RS26930 (OQE50_26930) 1709..2449 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
OQE50_RS26935 (OQE50_26935) 2813..3781 - 969 WP_023307223.1 IS5-like element IS903B family transposase -
OQE50_RS26940 (OQE50_26940) 4026..4229 + 204 WP_004213596.1 HHA domain-containing protein -
OQE50_RS26945 (OQE50_26945) 4243..4473 + 231 WP_023307222.1 hypothetical protein -
OQE50_RS26950 (OQE50_26950) 4549..5529 + 981 WP_000082736.1 IS5-like element ISEc68 family transposase -
OQE50_RS26955 (OQE50_26955) 6119..6385 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
OQE50_RS26960 (OQE50_26960) 6373..6855 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
OQE50_RS26965 (OQE50_26965) 7067..8410 + 1344 WP_080339306.1 ISNCY family transposase -
OQE50_RS26970 (OQE50_26970) 8481..9299 - 819 WP_023307220.1 abortive infection family protein -
OQE50_RS26975 (OQE50_26975) 9308..9880 - 573 WP_032676095.1 recombinase family protein -
OQE50_RS26980 (OQE50_26980) 10220..10924 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
OQE50_RS26985 (OQE50_26985) 10958..11216 + 259 Protein_12 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-10 - 1..118766 118766
- inside IScluster/Tn - - 2813..16563 13750


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T264425 WP_003026803.1 NZ_CP110881:6373-6855 [Enterobacter kobei]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT264425 WP_003026799.1 NZ_CP110881:6119-6385 [Enterobacter kobei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References