Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 581875..582451 Replicon chromosome
Accession NZ_CP110823
Organism Enterobacter roggenkampii strain WS22-2

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag OM419_RS02680 Protein ID WP_025912099.1
Coordinates 581875..582162 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7D6YIG8
Locus tag OM419_RS02685 Protein ID WP_025912101.1
Coordinates 582149..582451 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM419_RS02650 (OM419_02650) 576888..577004 + 117 Protein_511 amino acid-binding protein -
OM419_RS02655 (OM419_02655) 576997..577692 + 696 WP_039023948.1 winged helix-turn-helix domain-containing protein -
OM419_RS02660 (OM419_02660) 577838..578308 + 471 WP_126324966.1 MarR family transcriptional regulator -
OM419_RS02665 (OM419_02665) 578305..579372 + 1068 WP_277738499.1 HlyD family secretion protein -
OM419_RS02670 (OM419_02670) 579362..580438 + 1077 WP_094943938.1 DUF2955 domain-containing protein -
OM419_RS02675 (OM419_02675) 580435..581706 - 1272 WP_087450691.1 DUF445 domain-containing protein -
OM419_RS02680 (OM419_02680) 581875..582162 + 288 WP_025912099.1 BrnT family toxin Toxin
OM419_RS02685 (OM419_02685) 582149..582451 + 303 WP_025912101.1 BrnA antitoxin family protein Antitoxin
OM419_RS02690 (OM419_02690) 582482..583120 - 639 WP_277738147.1 LysE family translocator -
OM419_RS02695 (OM419_02695) 583168..583911 - 744 WP_059363112.1 AraC family transcriptional regulator -
OM419_RS02700 (OM419_02700) 584063..585433 + 1371 WP_059363111.1 NAD-dependent succinate-semialdehyde dehydrogenase -
OM419_RS02705 (OM419_02705) 585479..585799 - 321 WP_032660910.1 hypothetical protein -
OM419_RS02710 (OM419_02710) 585799..586344 - 546 WP_008501313.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T264357 WP_025912099.1 NZ_CP110823:581875-582162 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11413.02 Da        Isoelectric Point: 10.0592

>AT264357 WP_025912101.1 NZ_CP110823:582149-582451 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARREAELKALAEKPEAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A7D6YIG8

References