Detailed information of TA system TA026433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3166476..3168196
• Replicon: chromosome
• Accession: NC_017309
• Organism: Edwardsiella tarda FL6-60

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A411H3H5
• Locus tag: ETAF_RS13915
• Protein ID: WP_015683486.1
• Coordinates: 3167540..3168196 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: ETAF_RS13910
• Protein ID: WP_041692662.1
• Coordinates: 3166476..3167543 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETAF_RS13900	3162501..3163217	-	717	WP_012849890.1	M48 family metallopeptidase	-
ETAF_RS13905	3163217..3166312	-	3096	WP_012849889.1	HsdR family type I site-specific deoxyribonuclease	-
ETAF_RS13910	3166476..3167543	-	1068	WP_041692662.1	macro domain-containing protein	Antitoxin
ETAF_RS13915	3167540..3168196	-	657	WP_015683486.1	DUF4433 domain-containing protein	Toxin
ETAF_RS13920	3168209..3169519	-	1311	WP_071819616.1	restriction endonuclease subunit S	-
ETAF_RS13925	3169570..3172008	-	2439	WP_015683488.1	type I restriction-modification system subunit M	-
ETAF_RS13930	3172047..3172286	-	240	WP_012849883.1	hypothetical protein	-
ETAF_RS13935	3172638..3172994	-	357	Protein_2754	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25046.43 Da
• Isoelectric Point: 6.9045

>T26433 WP_015683486.1 NC_017309:c3168196-3167540 [Edwardsiella tarda FL6-60]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSQVQAVNWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSSLRSVAAQNAPFVFTDSHAYYNWANYYTRLEDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWQHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T26433 NC_017309:c3168196-3167540 [Edwardsiella tarda FL6-60]
ATGGCTTATGACTACAGCGCCAGTCTCAACCCGCAAAAAGCCCTGATCTGGCGCATTGTCCATCGGGATAATATCCCGTG
GATACTGGACAACGGCTTGCACTGCGGCAACAGTCAAGTGCAGGCGGTAAACTGGATCAATATCGGCAACCCGGAACTTA
TCGGCAAACGCGCCGGGCATCCGGTGCCTGTCGGGACGGGCGGGACGCTGCATGACTACGTGCCGTTTTACTTTACCCCC
TTTTCGCCGATGCTGATGAATATCCACAGCGGGCGTGGCGGCATTACGCAGCGCCCCAACGAGGAAATCGTCATTCTGGT
CAGCAGCCTGCGAAGCGTGGCGGCGCAAAACGCCCCCTTCGTGTTTACCGACAGCCATGCCTACTATAACTGGGCCAACT
ACTACACCCGCCTGGAGGACCTGAACCAGATCGACTGGTCTATCTTACAGGCGCGGGATTTCCGTCGCGATCCGGACGAT
CCGGCGAAGTTTGAACGCTATCAGGCCGAGGCGTTAATCTGGCAGCACTGCCCCATCACACTGCTGGTCGGGATGATTTG
TTACAACGATGGCGTCAGGTTACAATTAGAGCAGTGGCTGTCGCAACGTAATCTGACAATGCCCGTTCACGCACGGGCCG
GGTGGTACTTCTCATGA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40668.02 Da
• Isoelectric Point: 6.9067

>AT26433 WP_041692662.1 NC_017309:c3167543-3166476 [Edwardsiella tarda FL6-60]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYAHACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIELHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT26433 NC_017309:c3167543-3166476 [Edwardsiella tarda FL6-60]
ATGATCACATATACACAAGGCAATTTACTGGACGCGCCCGTGGAAGCACTGGTGAATACGGTGAATACCGTCGGTGTCAT
GGGTAAAGGCATCGCGCTGATGTTTAAAGAGCGCTTCCCCGACAATATGAAAGCCTATGCCCACGCCTGCAAACAAAAGC
AGGTGATGACCGGCAAGATGTTTATTACCGAAACCGGCGAGTTGATGGGGCCGCGCTGGATCGTCAACTTCCCTACCAAG
CAGCACTGGCGCGCCGACTCTCGCATGGCGTGGATCGAGGACGGCTTGCAGGACCTGCGCCGCTTTTTGATCGAAAAGAA
AGTGCGGTCCATCGCCATTCCCCCGCTGGGCGCGGGCAACGGCGGCCTGAACTGGCCGGACGTCCGGGCGCGGATTGAAT
CTGCGCTGGGCGACCTTCAGGACACGGAAATCCTGATTTATGAGCCTACCGAAAAATACCAGAACGTCGCCAAGAGCAGC
GGTGTAAAAAAGCTCACTCCCGCCAGAGCGATGATCGCTGAACTGGTGCGCCGCTACTGGGTGCTGGGCATGGAGTGCAG
CCTGCTGGAAATCCAGAAGCTGGCGTGGCTGCTACAACGCGCCATTGAACTGCACCAGCAGGAGGACGTGCTAAAACTGC
GGTTTGAGGCGCACTATTACGGCCCCTATGCTCCTAACCTGAACCATCTGCTTAACGCGCTGGACGGTACCTATCTGAAA
GCCGAAAAGCGTATCCCGGACAGCCAGCCACTGGATGTGATCTGGTTTAACGATCGGGAAAAAGAACACATCAACCTATG
GCTGAACAGCGAAGCCAAAGCGTGGTTGCCAGCGCTTGAGCAGGTCAGCCAGTTGATTGACGGTTTTGAATCGCCGTTTG
GTCTGGAGCTGTTGGCGACCGTTGACTGGTTGTTGACCCGGGGACAATGCCAGCCCACGCTGGACTCCGTGAAAGAGGGG
TTGCATCAGTGGCCTGCGGGAGAACCTTGGGCCAGCCGTAAGATAAAGCTGTTTGACGATAAGAATCTGCAATTCGCCAT
TAACCGCGTGATGGAGTTTCATTGCTGA

Structures

Toxin

• AlphaFold DB: A0A411H3H5