264310

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3523500..3524179 Replicon chromosome
Accession NZ_CP110814
Organism Escherichia coli strain THB42-F4

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag N0552_RS17175 Protein ID WP_000854672.1
Coordinates 3523500..3523841 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag N0552_RS17180 Protein ID WP_000070395.1
Coordinates 3523862..3524179 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0552_RS17150 (3519086) 3519086..3519178 + 93 Protein_3350 sigma factor-binding protein Crl -
N0552_RS17155 (3519217) 3519217..3520272 - 1056 WP_000749863.1 phosphoporin PhoE -
N0552_RS17160 (3520560) 3520560..3521663 + 1104 WP_001285288.1 glutamate 5-kinase -
N0552_RS17165 (3521675) 3521675..3522928 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
N0552_RS17175 (3523500) 3523500..3523841 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
N0552_RS17180 (3523862) 3523862..3524179 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
N0552_RS17185 (3524198) 3524198..3524419 - 222 WP_000691994.1 DUF987 domain-containing protein -
N0552_RS17190 (3524428) 3524428..3524904 - 477 WP_000811693.1 RadC family protein -
N0552_RS17195 (3524920) 3524920..3525378 - 459 WP_000211838.1 antirestriction protein -
N0552_RS17200 (3525476) 3525476..3525715 - 240 WP_000194654.1 DUF905 family protein -
N0552_RS17205 (3525792) 3525792..3526259 - 468 WP_001547765.1 protein YkfB -
N0552_RS17210 (3526282) 3526282..3526725 - 444 WP_000824223.1 lipoprotein YafY -
N0552_RS17215 (3526725) 3526725..3526901 - 177 WP_001285112.1 hypothetical protein -
N0552_RS17220 (3526948) 3526948..3527139 - 192 Protein_3363 DeoR family transcriptional regulator -
N0552_RS17225 (3527356) 3527356..3528177 - 822 WP_000197389.1 DUF932 domain-containing protein -
N0552_RS17230 (3528269) 3528269..3529132 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T264310 WP_000854672.1 NZ_CP110814:c3523841-3523500 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT264310 WP_000070395.1 NZ_CP110814:c3524179-3523862 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References