Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 92732..94300 Replicon plasmid pCf-VIM
Accession NZ_CP110776
Organism Citrobacter freundii strain Cf-Emp

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag OPR78_RS25855 Protein ID WP_024134513.1
Coordinates 92993..94300 (+) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag OPR78_RS25850 Protein ID WP_039272366.1
Coordinates 92732..92983 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPR78_RS25820 (OPR78_25810) 88385..89110 + 726 WP_001553093.1 hypothetical protein -
OPR78_RS25825 (OPR78_25815) 89166..89618 + 453 WP_001553092.1 hypothetical protein -
OPR78_RS25830 (OPR78_25820) 89634..90236 - 603 WP_039272372.1 hypothetical protein -
OPR78_RS25835 (OPR78_25825) 90497..90778 + 282 WP_000575656.1 hypothetical protein -
OPR78_RS25840 (OPR78_25830) 90797..91360 + 564 WP_001132404.1 hypothetical protein -
OPR78_RS25845 (OPR78_25835) 91363..92367 + 1005 WP_039272368.1 tyrosine-type recombinase/integrase -
OPR78_RS25850 (OPR78_25840) 92732..92983 + 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
OPR78_RS25855 (OPR78_25845) 92993..94300 + 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
OPR78_RS25860 (OPR78_25850) 94297..94791 + 495 WP_039272364.1 hypothetical protein -
OPR78_RS25865 (OPR78_25855) 94966..95904 - 939 WP_044596315.1 hypothetical protein -
OPR78_RS25870 (OPR78_25860) 96103..97743 + 1641 WP_031611419.1 PAAR domain-containing protein -
OPR78_RS25875 (OPR78_25865) 98115..98417 - 303 WP_031611417.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / mph(A) / blaSHV-12 / blaVIM-1 / aph(3')-XV / ant(3'')-Ia / catB2 / qacE / sul1 - 1..151674 151674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T264214 WP_024134513.1 NZ_CP110776:92993-94300 [Citrobacter freundii]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT264214 WP_039272366.1 NZ_CP110776:92732-92983 [Citrobacter freundii]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References