Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4357337..4358016 Replicon chromosome
Accession NZ_CP110775
Organism Citrobacter freundii strain Cf-Emp

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag OPR78_RS21490 Protein ID WP_003031349.1
Coordinates 4357337..4357678 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag OPR78_RS21495 Protein ID WP_003031347.1
Coordinates 4357699..4358016 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPR78_RS21465 (4352462) 4352462..4352815 + 354 WP_016239889.1 hypothetical protein -
OPR78_RS21470 (4352816) 4352816..4354015 + 1200 WP_121927013.1 baseplate J/gp47 family protein -
OPR78_RS21475 (4354012) 4354012..4354692 + 681 WP_121927014.1 DUF2612 domain-containing protein -
OPR78_RS21480 (4354692) 4354692..4356143 + 1452 WP_224770544.1 hypothetical protein -
OPR78_RS21485 (4356145) 4356145..4356747 + 603 WP_224770543.1 tail fiber assembly protein -
OPR78_RS21490 (4357337) 4357337..4357678 - 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
OPR78_RS21495 (4357699) 4357699..4358016 - 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
OPR78_RS21500 (4358034) 4358034..4358255 - 222 WP_003031346.1 DUF987 domain-containing protein -
OPR78_RS21505 (4358264) 4358264..4358740 - 477 WP_003031345.1 RadC family protein -
OPR78_RS21510 (4358756) 4358756..4359217 - 462 WP_003031344.1 antirestriction protein -
OPR78_RS21515 (4359926) 4359926..4361923 + 1998 WP_016245729.1 choline BCCT transporter BetT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4314880..4359217 44337


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T264212 WP_003031349.1 NZ_CP110775:c4357678-4357337 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT264212 WP_003031347.1 NZ_CP110775:c4358016-4357699 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References