264209

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3461410..3462026 Replicon chromosome
Accession NZ_CP110775
Organism Citrobacter freundii strain Cf-Emp

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag OPR78_RS17220 Protein ID WP_003028682.1
Coordinates 3461652..3462026 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag OPR78_RS17215 Protein ID WP_043018956.1
Coordinates 3461410..3461652 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPR78_RS17190 (3457260) 3457260..3457859 + 600 WP_016151258.1 glucose-1-phosphatase -
OPR78_RS17195 (3457853) 3457853..3458725 + 873 WP_003028669.1 virulence factor BrkB family protein -
OPR78_RS17200 (3458722) 3458722..3459159 + 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
OPR78_RS17205 (3459204) 3459204..3460145 + 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
OPR78_RS17210 (3460296) 3460296..3461204 - 909 WP_044701816.1 alpha/beta hydrolase -
OPR78_RS17215 (3461410) 3461410..3461652 + 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
OPR78_RS17220 (3461652) 3461652..3462026 + 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
OPR78_RS17225 (3462063) 3462063..3462992 - 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
OPR78_RS17230 (3462989) 3462989..3463624 - 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
OPR78_RS17235 (3463621) 3463621..3464523 - 903 WP_003028691.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T264209 WP_003028682.1 NZ_CP110775:3461652-3462026 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT264209 WP_043018956.1 NZ_CP110775:3461410-3461652 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References