Detailed information of TA system TA026420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 2004077..2004683
• Replicon: chromosome
• Accession: NC_017276
• Organism: Sulfolobus islandicus REY15A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M9UBU6
• Locus tag: SIRE_RS11035
• Protein ID: WP_014513042.1
• Coordinates: 2004279..2004683 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M9UBQ9
• Locus tag: SIRE_RS11030
• Protein ID: WP_009989091.1
• Coordinates: 2004077..2004298 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SIRE_RS11000 (SiRe_2164)	1999825..2000442	+	618	WP_012712231.1	hypothetical protein	-
SIRE_RS11005 (SiRe_2165)	2000439..2000825	+	387	WP_014513039.1	hypothetical protein	-
SIRE_RS11010 (SiRe_2166)	2000822..2001487	-	666	WP_014513040.1	helix-turn-helix domain-containing protein	-
SIRE_RS11015	2001557..2002191	-	635	Protein_2211	IS6 family transposase	-
SIRE_RS11020 (SiRe_2168)	2002336..2003607	-	1272	WP_014514197.1	MFS transporter	-
SIRE_RS11025 (SiRe_2169)	2003661..2003933	-	273	WP_048052822.1	EamA family transporter	-
SIRE_RS11030 (SiRe_2170)	2004077..2004298	+	222	WP_009989091.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SIRE_RS11035 (SiRe_2171)	2004279..2004683	+	405	WP_014513042.1	PIN domain-containing protein	Toxin
SIRE_RS14485	2004879..2005190	-	312	WP_274378338.1	transposase	-
SIRE_RS14490	2005211..2005318	+	108	Protein_2217	IS110 family transposase	-
SIRE_RS11045 (SiRe_2172)	2005848..2006627	-	780	WP_014511459.1	DMT family transporter	-
SIRE_RS11050 (SiRe_2173)	2007461..2007643	+	183	WP_009990866.1	hypothetical protein	-
SIRE_RS11055 (SiRe_2174)	2007791..2008552	+	762	WP_009990867.1	ZIP family metal transporter	-
SIRE_RS11060 (SiRe_2175)	2008590..2009498	+	909	WP_014514199.1	dihydrodipicolinate synthase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2001557..2005064	3507

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (4-48)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15441.05 Da
• Isoelectric Point: 5.2947

>T26420 WP_014513042.1 NC_017276:2004279-2004683 [Sulfolobus islandicus REY15A]
VEKAIVDTNVIIYDYVEDSEYHKRAEELLDSLDRWVIPVIVVHEIMWFLKGMKLEDKVDDVLAYIRSEKAEVVCDCTDNV
TDAIGVLKKEKLPLVSYKDMVILSHAIREKLPLATFDRKLSKIAKKYSVSLLQS

Nucleotide

• Length: 405 bp

>T26420 NC_017276:2004279-2004683 [Sulfolobus islandicus REY15A]
GTGGAGAAGGCAATAGTTGACACTAACGTGATAATTTACGACTACGTTGAAGACTCCGAATATCATAAGAGGGCGGAAGA
GCTTCTCGATAGTTTGGATAGGTGGGTAATTCCCGTGATTGTAGTTCATGAGATCATGTGGTTCTTAAAGGGCATGAAAC
TTGAAGACAAGGTAGATGACGTTTTGGCTTATATTAGAAGTGAGAAAGCTGAGGTTGTCTGCGATTGTACAGATAACGTC
ACTGACGCTATAGGAGTGCTGAAGAAGGAAAAGCTTCCCTTGGTCAGCTACAAAGATATGGTAATACTTTCTCACGCAAT
TAGAGAAAAATTGCCTTTAGCCACTTTTGATAGGAAACTCTCAAAGATAGCAAAGAAGTACTCCGTAAGTTTGCTACAGT
CGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8267.50 Da
• Isoelectric Point: 9.6908

>AT26420 WP_009989091.1 NC_017276:2004077-2004298 [Sulfolobus islandicus REY15A]
MERVKVTRNYQITIPASARSKINIKEGDILEVYLNGDEIVLRKVKTERPRIRLGSKLSLGDIEEAIERGEGNS

Nucleotide

• Length: 222 bp

>AT26420 NC_017276:2004077-2004298 [Sulfolobus islandicus REY15A]
ATGGAGAGGGTAAAGGTTACGAGAAATTACCAGATAACTATTCCAGCCTCGGCTAGGAGTAAGATTAATATAAAAGAAGG
AGATATACTTGAGGTTTACTTGAACGGTGACGAGATAGTACTTCGGAAGGTTAAGACTGAGAGGCCGAGGATAAGGCTAG
GCAGTAAACTCTCACTGGGAGATATTGAGGAGGCAATAGAGCGTGGAGAAGGCAATAGTTGA

Structures

Toxin

• AlphaFold DB: M9UBU6

Antitoxin

• AlphaFold DB: M9UBQ9