Detailed information of TA system TA264180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3658625..3659165
• Replicon: chromosome
• Accession: NZ_CP110674
• Organism: Mycobacterium tuberculosis strain BLR-31d

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII0
• Locus tag: OO115_RS17255
• Protein ID: WP_003403376.1
• Coordinates: 3658857..3659165 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: OO115_RS17250
• Protein ID: WP_003403381.1
• Coordinates: 3658625..3658870 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO115_RS17220 (OO115_17220)	3654541..3654714	-	174	WP_003898549.1	hypothetical protein	-
OO115_RS17225 (OO115_17225)	3654711..3655049	-	339	WP_003403405.1	PIN domain-containing protein	-
OO115_RS17230 (OO115_17230)	3654961..3655287	-	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
OO115_RS17235 (OO115_17235)	3655350..3657713	-	2364	WP_003901895.1	arylsulfatase AtsD	-
OO115_RS17240 (OO115_17240)	3657827..3658081	+	255	WP_003911263.1	antitoxin VapB7	-
OO115_RS17245 (OO115_17245)	3658078..3658515	+	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
OO115_RS17250 (OO115_17250)	3658625..3658870	+	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
OO115_RS17255 (OO115_17255)	3658857..3659165	+	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OO115_RS17260 (OO115_17260)	3659441..3660157	+	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
OO115_RS17265 (OO115_17265)	3660233..3660388	+	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
OO115_RS17270 (OO115_17270)	3660483..3660866	+	384	WP_003403365.1	ribonuclease VapC6	-
OO115_RS17275 (OO115_17275)	3661254..3662264	-	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
OO115_RS17280 (OO115_17280)	3662345..3663850	-	1506	WP_003403360.1	carotenoid cleavage oxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.10 Da
• Isoelectric Point: 11.6554

>T264180 WP_003403376.1 NZ_CP110674:3658857-3659165 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T264180 NZ_CP110674:3658857-3659165 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT264180 WP_003403381.1 NZ_CP110674:3658625-3658870 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT264180 NZ_CP110674:3658625-3658870 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSE5

Antitoxin

• AlphaFold DB: G0TQE0