Detailed information of TA system TA026416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 996048..996654
• Replicon: chromosome
• Accession: NC_017275
• Organism: Sulfolobus islandicus HVE10/4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M9U4G3
• Locus tag: SIH_RS05280
• Protein ID: WP_012710548.1
• Coordinates: 996292..996654 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M9UCL5
• Locus tag: SIH_RS05275
• Protein ID: WP_012718013.1
• Coordinates: 996048..996269 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SIH_RS05235 (SiH_0980)	991213..991629	+	417	WP_014512464.1	hypothetical protein	-
SIH_RS05240 (SiH_0981)	991610..992212	+	603	WP_014512465.1	urease accessory UreF family protein	-
SIH_RS05245 (SiH_0982)	992209..992814	+	606	WP_014512466.1	urease accessory protein UreG	-
SIH_RS05250 (SiH_0983)	992826..993407	+	582	WP_237702661.1	urease accessory protein UreD	-
SIH_RS15525 (SiH_0984)	993454..993588	+	135	WP_014512468.1	hypothetical protein	-
SIH_RS15190	994660..994824	+	165	WP_193385925.1	hypothetical protein	-
SIH_RS05270 (SiH_0986)	994811..995905	+	1095	WP_014512470.1	hypothetical protein	-
SIH_RS05275 (SiH_0987)	996048..996269	+	222	WP_012718013.1	hypothetical protein	Antitoxin
SIH_RS05280 (SiH_0988)	996292..996654	+	363	WP_012710548.1	type II toxin-antitoxin system VapC family toxin	Toxin
SIH_RS05285 (SiH_0989)	996782..997987	-	1206	WP_052891907.1	RNA-guided endonuclease TnpB family protein	-
SIH_RS05290 (SiH_0990)	998432..999985	-	1554	WP_014512472.1	aldehyde dehydrogenase family protein	-
SIH_RS05295 (SiH_0991)	1000077..1000670	+	594	WP_012718017.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	996782..998035	1253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (21-113)

Antitoxin

• Putative antitoxin VapB3-like (1-73)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13870.21 Da
• Isoelectric Point: 9.2430

>T26416 WP_012710548.1 NC_017275:996292-996654 [Sulfolobus islandicus HVE10/4]
VTKEKGWNRITELLKDSETLDLALVEVSNVIWKKVVLTNEITPQDANKAIMIIKEYLPQLLTVNKSVKLLERAIDISIRE
KLTIYDSLYIALAESKRSKLLTSDKKQYEVARKYVDSELF

Nucleotide

• Length: 363 bp

>T26416 NC_017275:996292-996654 [Sulfolobus islandicus HVE10/4]
GTGACTAAGGAGAAGGGATGGAATAGAATTACGGAGTTACTAAAGGATAGTGAAACTCTAGATCTGGCGCTAGTAGAAGT
TTCCAATGTTATTTGGAAGAAAGTGGTGTTAACAAACGAGATTACTCCACAGGATGCCAATAAGGCGATTATGATTATTA
AGGAATATCTACCTCAATTGCTGACTGTAAATAAAAGCGTTAAACTCTTGGAAAGAGCTATAGATATCTCTATAAGGGAA
AAACTGACTATATACGACTCGCTATATATTGCCTTAGCGGAAAGCAAGAGAAGTAAATTATTAACCAGCGATAAAAAGCA
ATATGAGGTTGCAAGAAAATACGTGGACTCGGAATTATTTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8952.22 Da
• Isoelectric Point: 4.6475

>AT26416 WP_012718013.1 NC_017275:996048-996269 [Sulfolobus islandicus HVE10/4]
MKMVYSLRIDKELREEMEKYDIKWNEEIEGFIRKRLEELKKEEILKRINEILSTMPETNSSSAEMVREDRDNN

Nucleotide

• Length: 222 bp

>AT26416 NC_017275:996048-996269 [Sulfolobus islandicus HVE10/4]
ATGAAAATGGTATACAGTTTAAGGATAGATAAAGAATTACGTGAGGAGATGGAAAAGTACGACATAAAGTGGAATGAAGA
GATAGAGGGTTTCATAAGGAAAAGGCTTGAGGAATTGAAAAAAGAGGAGATTCTAAAGAGGATTAATGAGATCCTTTCAA
CCATGCCGGAAACTAATTCAAGTAGTGCGGAAATGGTGAGGGAAGATAGGGATAATAATTGA

Structures

Toxin

• AlphaFold DB: M9U4G3

Antitoxin

• AlphaFold DB: M9UCL5