Detailed information of TA system TA026412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 289716..290437
• Replicon: plasmid pTHTHE1601
• Accession: NC_017273
• Organism: Thermus thermophilus SG0.5JP17-16

Toxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: THTHE16_RS11485
• Protein ID: WP_014511247.1
• Coordinates: 289716..290132 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: THTHE16_RS11490
• Protein ID: WP_014511248.1
• Coordinates: 290129..290437 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THTHE16_RS11460	284900..285949	+	1050	WP_014511242.1	galactose-1-phosphate uridylyltransferase	-
THTHE16_RS11465	285968..286621	+	654	Protein_289	DUF1722 domain-containing protein	-
THTHE16_RS11470	286652..287044	-	393	WP_014511244.1	nucleotidyltransferase domain-containing protein	-
THTHE16_RS11475	287054..288052	-	999	WP_014511245.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
THTHE16_RS11480	288049..289617	-	1569	WP_014511246.1	phytoene desaturase	-
THTHE16_RS11485	289716..290132	-	417	WP_014511247.1	type II toxin-antitoxin system VapC family toxin	Toxin
THTHE16_RS11490	290129..290437	-	309	WP_014511248.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
THTHE16_RS11495	290443..291465	-	1023	WP_014511249.1	glycosyltransferase	-
THTHE16_RS11500	291462..292136	-	675	WP_014511250.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
THTHE16_RS11505	292141..292839	-	699	WP_014511251.1	lycopene cyclase domain-containing protein	-
THTHE16_RS11510	292836..294005	-	1170	WP_014511252.1	cytochrome P450	-
THTHE16_RS11515	294015..294194	-	180	WP_014511253.1	hypothetical protein	-
THTHE16_RS11520	294240..295217	-	978	Protein_300	deoxyribodipyrimidine photo-lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..440026	440026

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-129)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (20-83)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15241.82 Da
• Isoelectric Point: 10.4393

>T26412 WP_014511247.1 NC_017273:c290132-289716 [Thermus thermophilus SG0.5JP17-16]
MRYLLDTNVVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAE
VMELWGELTGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 417 bp

>T26412 NC_017273:c290132-289716 [Thermus thermophilus SG0.5JP17-16]
CTGAGGTACCTGCTGGACACCAACGTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCG
CCACCTTAAAGTGGGGGAAGCGTACCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGG
CCCGAAGGGCCATCCTCAGCCGATGGCTCTCGGGGCTGAAGGCGAGCTTCTCTGGGCGGATCCTTCCCCTGGACGCCGAG
GTGATGGAGCTCTGGGGCGAGCTCACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGC
GGCCACGGCCCTGCGGCACGGCCTGATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGA
ACCCCTGGGAAGGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11641.34 Da
• Isoelectric Point: 5.2809

>AT26412 WP_014511248.1 NC_017273:c290437-290129 [Thermus thermophilus SG0.5JP17-16]
VASELDRPVFSVYPEGVKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKT
LPNEEVEALFQRQKTGFREVDF

Nucleotide

• Length: 309 bp

>AT26412 NC_017273:c290437-290129 [Thermus thermophilus SG0.5JP17-16]
GTGGCCTCTGAACTAGACAGACCGGTCTTTTCCGTTTACCCTGAGGGGGTGAAGGCCATCTGGCAACTCCAAGAGGCCAA
AGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCAGCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGG
TGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCTCGGTCCTCGAGGCCCTAAGACCCCCCAAGACC
CTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGCTTCCGGGAGGTGGACTTCTGA