Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4285467..4286092 Replicon chromosome
Accession NZ_CP110661
Organism Klebsiella pneumoniae strain B431

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OOS97_RS20960 Protein ID WP_002882817.1
Coordinates 4285709..4286092 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OOS97_RS20955 Protein ID WP_004150355.1
Coordinates 4285467..4285709 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OOS97_RS20930 (4281458) 4281458..4282057 + 600 WP_004151865.1 glucose-1-phosphatase -
OOS97_RS20935 (4282051) 4282051..4282911 + 861 WP_004146232.1 virulence factor BrkB family protein -
OOS97_RS20940 (4282908) 4282908..4283345 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OOS97_RS20945 (4283390) 4283390..4284331 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OOS97_RS20950 (4284345) 4284345..4285262 - 918 WP_023302328.1 alpha/beta hydrolase -
OOS97_RS20955 (4285467) 4285467..4285709 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OOS97_RS20960 (4285709) 4285709..4286092 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OOS97_RS20965 (4286266) 4286266..4287195 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OOS97_RS20970 (4287192) 4287192..4287827 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OOS97_RS20975 (4287824) 4287824..4288726 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T264110 WP_002882817.1 NZ_CP110661:4285709-4286092 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT264110 WP_004150355.1 NZ_CP110661:4285467-4285709 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References