Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4787203..4788013 Replicon chromosome
Accession NZ_CP110638
Organism Klebsiella pneumoniae strain K201054

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag OO068_RS24450 Protein ID WP_004178461.1
Coordinates 4787203..4787736 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OO068_RS24455 Protein ID WP_002887278.1
Coordinates 4787747..4788013 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO068_RS24445 4786034..4787155 + 1122 WP_064153030.1 cupin domain-containing protein -
OO068_RS24450 4787203..4787736 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
OO068_RS24455 4787747..4788013 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OO068_RS24460 4788116..4789549 - 1434 WP_009484674.1 Cu(+)/Ag(+) sensor histidine kinase -
OO068_RS24465 4789539..4790222 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
OO068_RS24470 4790395..4791780 + 1386 WP_064153031.1 efflux transporter outer membrane subunit -
OO068_RS24475 4791798..4792142 + 345 WP_048266266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T264064 WP_004178461.1 NZ_CP110638:c4787736-4787203 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT264064 WP_002887278.1 NZ_CP110638:c4788013-4787747 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References