Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 571735..572371 Replicon chromosome
Accession NZ_CP110634
Organism Bacillus subtilis strain MG-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OOZ27_RS03015 Protein ID WP_003156187.1
Coordinates 572021..572371 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag OOZ27_RS03010 Protein ID WP_003225183.1
Coordinates 571735..572016 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OOZ27_RS02990 (568094) 568094..568693 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
OOZ27_RS02995 (568788) 568788..569153 + 366 WP_003234281.1 holo-ACP synthase -
OOZ27_RS03000 (569319) 569319..570335 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
OOZ27_RS03005 (570450) 570450..571619 + 1170 WP_003234284.1 alanine racemase -
OOZ27_RS03010 (571735) 571735..572016 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OOZ27_RS03015 (572021) 572021..572371 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OOZ27_RS03020 (572486) 572486..573310 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
OOZ27_RS03025 (573315) 573315..573680 + 366 WP_015715277.1 RsbT antagonist protein RsbS -
OOZ27_RS03030 (573684) 573684..574085 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
OOZ27_RS03035 (574097) 574097..575104 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
OOZ27_RS03040 (575166) 575166..575495 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
OOZ27_RS03045 (575492) 575492..575974 + 483 WP_003234299.1 anti-sigma B factor RsbW -
OOZ27_RS03050 (575940) 575940..576728 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
OOZ27_RS03055 (576728) 576728..577327 + 600 WP_265392660.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T264037 WP_003156187.1 NZ_CP110634:572021-572371 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT264037 WP_003225183.1 NZ_CP110634:571735-572016 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References