Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 580733..581309 Replicon chromosome
Accession NZ_CP110534
Organism Enterobacter roggenkampii strain WS28-4

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag OM420_RS02700 Protein ID WP_025912099.1
Coordinates 580733..581020 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7D6YIG8
Locus tag OM420_RS02705 Protein ID WP_025912101.1
Coordinates 581007..581309 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM420_RS02670 (OM420_02670) 575746..575862 + 117 Protein_515 amino acid-binding protein -
OM420_RS02675 (OM420_02675) 575855..576550 + 696 WP_094943935.1 winged helix-turn-helix domain-containing protein -
OM420_RS02680 (OM420_02680) 576696..577166 + 471 WP_008501325.1 MarR family transcriptional regulator -
OM420_RS02685 (OM420_02685) 577163..578230 + 1068 WP_025912094.1 HlyD family secretion protein -
OM420_RS02690 (OM420_02690) 578220..579296 + 1077 WP_094943938.1 DUF2955 domain-containing protein -
OM420_RS02695 (OM420_02695) 579293..580564 - 1272 WP_087450691.1 DUF445 domain-containing protein -
OM420_RS02700 (OM420_02700) 580733..581020 + 288 WP_025912099.1 BrnT family toxin Toxin
OM420_RS02705 (OM420_02705) 581007..581309 + 303 WP_025912101.1 BrnA antitoxin family protein Antitoxin
OM420_RS02710 (OM420_02710) 581340..581978 - 639 WP_025912103.1 LysE family translocator -
OM420_RS02715 (OM420_02715) 582026..582769 - 744 WP_094943941.1 AraC family transcriptional regulator -
OM420_RS02720 (OM420_02720) 582921..584291 + 1371 WP_094943944.1 NAD-dependent succinate-semialdehyde dehydrogenase -
OM420_RS02725 (OM420_02725) 584336..584656 - 321 WP_008501314.1 hypothetical protein -
OM420_RS02730 (OM420_02730) 584656..585201 - 546 WP_094943947.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T263941 WP_025912099.1 NZ_CP110534:580733-581020 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11413.02 Da        Isoelectric Point: 10.0592

>AT263941 WP_025912101.1 NZ_CP110534:581007-581309 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARREAELKALAEKPEAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A7D6YIG8

References