Detailed information of TA system TA026391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 943108..943667
• Replicon: chromosome
• Accession: NC_017251
• Organism: Brucella suis 1330

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BS1330_RS04530
• Protein ID: WP_004688321.1
• Coordinates: 943422..943667 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BS1330_RS04525
• Protein ID: WP_002964097.1
• Coordinates: 943108..943422 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS1330_RS04480	938798..939001	-	204	WP_004690812.1	hypothetical protein	-
BS1330_RS04485	939004..939219	-	216	WP_002971459.1	hypothetical protein	-
BS1330_RS04490	939216..939419	-	204	WP_002964088.1	hypothetical protein	-
BS1330_RS04495	939469..940188	+	720	WP_006072696.1	hypothetical protein	-
BS1330_RS04500	940185..940415	+	231	WP_002964091.1	hypothetical protein	-
BS1330_RS04505	940412..940687	+	276	WP_002964092.1	hypothetical protein	-
BS1330_RS04510	940710..941297	+	588	WP_004683738.1	hypothetical protein	-
BS1330_RS04515	941532..942242	+	711	WP_004683739.1	porin family protein	-
BS1330_RS04520	942590..942760	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BS1330_RS04525	943108..943422	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BS1330_RS04530	943422..943667	-	246	WP_004688321.1	BrnT family toxin	Toxin
BS1330_RS04535	943800..944438	+	639	Protein_890	tRNA-guanine transglycosylase	-
BS1330_RS17280	944508..945266	+	759	Protein_891	IS5 family transposase	-
BS1330_RS04545	945267..946040	+	774	WP_006132649.1	hypothetical protein	-
BS1330_RS04550	946175..947821	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	937026..951137	14111
	-	flank	IS/Tn	-	-	944508..944876	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T26391 WP_004688321.1 NC_017251:c943667-943422 [Brucella suis 1330]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T26391 NC_017251:c943667-943422 [Brucella suis 1330]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT26391 WP_002964097.1 NC_017251:c943422-943108 [Brucella suis 1330]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT26391 NC_017251:c943422-943108 [Brucella suis 1330]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA