Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 90819..92442 Replicon plasmid pRWS291.s2
Accession NZ_CP110516
Organism Klebsiella pneumoniae strain WS_29-1

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag OM421_RS27700 Protein ID WP_000088046.1
Coordinates 91117..92442 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag OM421_RS27695 Protein ID WP_024136327.1
Coordinates 90819..91112 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM421_RS27660 (OM421_27650) 85889..86020 + 132 WP_000374059.1 hypothetical protein -
OM421_RS27665 (OM421_27655) 86361..86642 - 282 WP_000077926.1 hypothetical protein -
OM421_RS27670 (OM421_27660) 86692..86883 - 192 WP_000398480.1 hypothetical protein -
OM421_RS27675 (OM421_27665) 87107..88279 + 1173 WP_000952349.1 IS21-like element IS21 family transposase -
OM421_RS27680 (OM421_27670) 88279..89076 + 798 WP_000544828.1 IS21-like element IS21 family helper ATPase IstB -
OM421_RS27685 (OM421_27675) 89115..89450 - 336 WP_065311125.1 hypothetical protein -
OM421_RS27690 (OM421_27680) 89823..90215 + 393 WP_000341066.1 hypothetical protein -
OM421_RS27695 (OM421_27685) 90819..91112 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
OM421_RS27700 (OM421_27690) 91117..92442 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
OM421_RS27705 (OM421_27695) 92503..92709 + 207 WP_000134171.1 hypothetical protein -
OM421_RS27710 (OM421_27700) 92811..93221 + 411 WP_000985911.1 hypothetical protein -
OM421_RS27715 (OM421_27705) 93234..94049 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
OM421_RS27720 (OM421_27710) 94303..94728 + 426 WP_001043843.1 hypothetical protein -
OM421_RS27725 (OM421_27715) 95277..95585 + 309 WP_001224687.1 hypothetical protein -
OM421_RS27730 (OM421_27720) 95601..96458 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
OM421_RS27735 (OM421_27725) 96520..96723 + 204 WP_001194555.1 hypothetical protein -
OM421_RS27740 (OM421_27730) 97065..97421 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-1.1 / aph(3')-Ia / tet(M) / aac(3)-IId / dfrA5 / qacE / sul1 - 1..223870 223870


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T263887 WP_000088046.1 NZ_CP110516:91117-92442 [Klebsiella pneumoniae]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT263887 WP_024136327.1 NZ_CP110516:90819-91112 [Klebsiella pneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References