Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 123254..123897 Replicon plasmid pRWS291.s1
Accession NZ_CP110515
Organism Klebsiella pneumoniae strain WS_29-1

Toxin (Protein)


Gene name vagD Uniprot ID A0A5P6KDG5
Locus tag OM421_RS26050 Protein ID WP_021312768.1
Coordinates 123254..123670 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5P6KGW8
Locus tag OM421_RS26055 Protein ID WP_021312767.1
Coordinates 123667..123897 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM421_RS26030 (OM421_26020) 119554..120018 + 465 WP_001243598.1 isoprenylcysteine carboxylmethyltransferase family protein -
OM421_RS26035 (OM421_26025) 120298..120822 - 525 Protein_131 IS5-like element ISKpn26 family transposase -
OM421_RS26040 (OM421_26030) 120858..122297 - 1440 Protein_132 IS66 family transposase -
OM421_RS26045 (OM421_26035) 122362..123066 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
OM421_RS26050 (OM421_26040) 123254..123670 - 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin Toxin
OM421_RS26055 (OM421_26045) 123667..123897 - 231 WP_021312767.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
OM421_RS26060 (OM421_26050) 124687..125610 - 924 WP_040120127.1 IS5 family transposase -
OM421_RS26065 (OM421_26055) 125689..126456 + 768 Protein_137 sugar-binding domain-containing protein -
OM421_RS26070 (OM421_26060) 126893..127651 + 759 WP_040120128.1 3-oxoacyl-ACP reductase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..361178 361178
- inside IScluster/Tn - - 120298..125610 5312


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15175.60 Da        Isoelectric Point: 7.2452

>T263881 WP_021312768.1 NZ_CP110515:c123670-123254 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8722.81 Da        Isoelectric Point: 4.4692

>AT263881 WP_021312767.1 NZ_CP110515:c123897-123667 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P6KDG5

Antitoxin

Source ID Structure
AlphaFold DB A0A5P6KGW8

References