Detailed information of TA system TA026388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 958438..958997
• Replicon: chromosome
• Accession: NC_017248
• Organism: Brucella melitensis NI

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: BMNI_RS04655
• Protein ID: WP_002966807.1
• Coordinates: 958752..958997 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: BMNI_RS04650
• Protein ID: WP_004683740.1
• Coordinates: 958438..958752 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMNI_RS04605	954143..954346	-	204	WP_004683737.1	hypothetical protein	-
BMNI_RS04610	954349..954564	-	216	WP_005968947.1	hypothetical protein	-
BMNI_RS04615	954561..954764	-	204	WP_002964088.1	hypothetical protein	-
BMNI_RS04620	954814..955518	+	705	WP_005968944.1	hypothetical protein	-
BMNI_RS04625	955515..955745	+	231	WP_002964091.1	hypothetical protein	-
BMNI_RS04630	955742..956017	+	276	WP_002964092.1	hypothetical protein	-
BMNI_RS04635	956040..956516	+	477	WP_002966806.1	hypothetical protein	-
BMNI_RS04640	956862..957572	+	711	WP_004683739.1	porin family protein	-
BMNI_RS04645	957920..958090	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BMNI_RS04650	958438..958752	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
BMNI_RS04655	958752..958997	-	246	WP_002966807.1	BrnT family toxin	Toxin
BMNI_RS04660	959130..959768	+	639	Protein_918	tRNA-guanine transglycosylase	-
BMNI_RS16435	959838..960464	+	627	Protein_919	IS5 family transposase	-
BMNI_RS04675	960465..961157	+	693	WP_006135935.1	hypothetical protein	-
BMNI_RS04680	961569..963020	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	952371..966336	13965
	-	flank	IS/Tn	-	-	959838..960206	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T26388 WP_002966807.1 NC_017248:c958997-958752 [Brucella melitensis NI]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T26388 NC_017248:c958997-958752 [Brucella melitensis NI]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT26388 WP_004683740.1 NC_017248:c958752-958438 [Brucella melitensis NI]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT26388 NC_017248:c958752-958438 [Brucella melitensis NI]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1