Detailed information of TA system TA026386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 961861..962420
• Replicon: chromosome
• Accession: NC_017244
• Organism: Brucella melitensis M28

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: BM28_RS04695
• Protein ID: WP_002966807.1
• Coordinates: 962175..962420 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: BM28_RS04690
• Protein ID: WP_004683740.1
• Coordinates: 961861..962175 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BM28_RS04640	957566..957769	-	204	WP_004683737.1	hypothetical protein	-
BM28_RS04645	957772..957987	-	216	WP_005968947.1	hypothetical protein	-
BM28_RS04650	957984..958187	-	204	WP_002964088.1	hypothetical protein	-
BM28_RS04655	958237..958941	+	705	WP_005968944.1	hypothetical protein	-
BM28_RS04660	958938..959168	+	231	WP_002964091.1	hypothetical protein	-
BM28_RS04665	959165..959440	+	276	WP_002964092.1	hypothetical protein	-
BM28_RS04670	959463..959939	+	477	WP_002966806.1	hypothetical protein	-
BM28_RS04675	960285..960995	+	711	WP_004683739.1	porin family protein	-
BM28_RS04680	961343..961513	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BM28_RS04685	961512..961745	+	234	WP_002969999.1	hypothetical protein	-
BM28_RS04690	961861..962175	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
BM28_RS04695	962175..962420	-	246	WP_002966807.1	BrnT family toxin	Toxin
BM28_RS04700	962553..963191	+	639	Protein_924	tRNA-guanine transglycosylase	-
BM28_RS17625	963261..964019	+	759	Protein_925	IS5 family transposase	-
BM28_RS04710	964020..964712	+	693	WP_006135935.1	hypothetical protein	-
BM28_RS04715	965124..966575	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	955794..969891	14097
	-	flank	IS/Tn	-	-	963261..963629	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T26386 WP_002966807.1 NC_017244:c962420-962175 [Brucella melitensis M28]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T26386 NC_017244:c962420-962175 [Brucella melitensis M28]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT26386 WP_004683740.1 NC_017244:c962175-961861 [Brucella melitensis M28]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT26386 NC_017244:c962175-961861 [Brucella melitensis M28]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1