263848

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2345090..2345666 Replicon chromosome
Accession NZ_CP110511
Organism Escherichia coli strain ws5-3

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag OM087_RS11265 Protein ID WP_001350372.1
Coordinates 2345379..2345666 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag OM087_RS11260 Protein ID WP_001402821.1
Coordinates 2345090..2345392 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM087_RS11240 (2340782) 2340782..2341294 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
OM087_RS11245 (2341686) 2341686..2343836 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
OM087_RS11250 (2343886) 2343886..2344089 + 204 WP_000467859.1 YbdD/YjiX family protein -
OM087_RS11255 (2344100) 2344100..2345056 + 957 WP_115724177.1 GTPase -
OM087_RS11260 (2345090) 2345090..2345392 - 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
OM087_RS11265 (2345379) 2345379..2345666 - 288 WP_001350372.1 BrnT family toxin Toxin
OM087_RS11270 (2345863) 2345863..2347029 + 1167 WP_024009509.1 restriction endonuclease -
OM087_RS11275 (2347093) 2347093..2348712 + 1620 WP_115724178.1 class I SAM-dependent DNA methyltransferase -
OM087_RS11280 (2348702) 2348702..2350051 + 1350 WP_115724179.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T263848 WP_001350372.1 NZ_CP110511:c2345666-2345379 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT263848 WP_001402821.1 NZ_CP110511:c2345392-2345090 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References