Detailed information of TA system TA026383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 969675..970323
• Replicon: chromosome
• Accession: NC_017221
• Organism: Bifidobacterium longum subsp. longum KACC 91563

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F8APW6
• Locus tag: BLNIAS_RS03935
• Protein ID: WP_014485576.1
• Coordinates: 969964..970323 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: F8APW5
• Locus tag: BLNIAS_RS03930
• Protein ID: WP_014485575.1
• Coordinates: 969675..969971 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLNIAS_RS03910	964770..965285	-	516	WP_041473707.1	IS3 family transposase	-
BLNIAS_RS03915	965382..965921	-	540	WP_008783376.1	peptidylprolyl isomerase	-
BLNIAS_RS03920	966919..968412	+	1494	WP_173362156.1	IS21 family transposase	-
BLNIAS_RS03925	968409..969170	+	762	WP_007054865.1	IS21-like element ISBlo2 family helper ATPase IstB	-
BLNIAS_RS03930	969675..969971	+	297	WP_014485575.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLNIAS_RS03935	969964..970323	+	360	WP_014485576.1	hypothetical protein	Toxin
BLNIAS_RS03940	970552..970962	-	411	Protein_769	protein-(glutamine-N5) methyltransferase	-
BLNIAS_RS03945	971159..972694	-	1536	WP_014485578.1	cell division protein FtsZ	-
BLNIAS_RS03950	972742..973464	-	723	WP_014485579.1	hypothetical protein	-
BLNIAS_RS03955	973561..974466	+	906	WP_014485580.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	959518..974466	14948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-52)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13428.24 Da
• Isoelectric Point: 6.2247

>T26383 WP_014485576.1 NC_017221:969964-970323 [Bifidobacterium longum subsp. longum KACC 91563]
MHEPEVYDVWLMRVEFPDHPGVGKIRPVIVTRVEGDRVSGIVVKVTSVTTWNGPGDVPLLDWRQEGLYKPSVARCSQRFW
FGDGDLLKYFGRLSLRDANDLDAGLDATGDIPPHRQSRP

Nucleotide

• Length: 360 bp

>T26383 NC_017221:969964-970323 [Bifidobacterium longum subsp. longum KACC 91563]
ATGCATGAGCCCGAAGTATATGACGTGTGGCTGATGCGCGTCGAGTTCCCCGATCATCCCGGCGTGGGCAAGATCAGGCC
CGTCATCGTCACCAGGGTCGAGGGCGATCGGGTCTCGGGTATCGTCGTCAAGGTGACCAGTGTGACGACATGGAACGGTC
CGGGTGACGTGCCGTTGCTTGACTGGCGTCAGGAGGGCCTATACAAGCCATCCGTGGCGAGATGCTCGCAGCGTTTCTGG
TTTGGCGATGGTGACTTGTTGAAGTATTTCGGCAGGTTGTCGTTGCGCGACGCGAACGACCTTGATGCCGGTCTTGATGC
GACGGGTGACATTCCGCCTCACCGTCAGTCGCGTCCATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11007.35 Da
• Isoelectric Point: 4.6556

>AT26383 WP_014485575.1 NC_017221:969675-969971 [Bifidobacterium longum subsp. longum KACC 91563]
MAVKERTTIALDPEVKREAADVLEQMGLSLSSFTEICLRQVVRDKGMPFTPSLRATRSDGYPVPPAHDAYRFERSKSTGN
IVLPQDWDDPADDIYDHA

Nucleotide

• Length: 297 bp

>AT26383 NC_017221:969675-969971 [Bifidobacterium longum subsp. longum KACC 91563]
ATGGCTGTGAAGGAACGGACGACCATTGCATTGGATCCCGAGGTCAAGCGTGAGGCGGCGGACGTGCTTGAGCAGATGGG
TTTGAGCCTGAGCTCATTCACGGAGATCTGTCTGCGGCAGGTGGTCCGCGACAAGGGCATGCCGTTCACACCGAGTCTGA
GGGCGACCCGATCCGATGGATATCCGGTTCCGCCGGCTCATGACGCGTATCGGTTCGAGCGTTCGAAGAGTACGGGCAAC
ATCGTACTGCCTCAGGACTGGGACGATCCGGCGGACGACATCTACGATCATGCATGA

Structures

Toxin

• AlphaFold DB: F8APW6

Antitoxin

• AlphaFold DB: F8APW5