Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1168274..1168829 Replicon chromosome
Accession NC_017218
Organism Bifidobacterium breve ACS-071-V-Sch8b

Toxin (Protein)


Gene name mazF Uniprot ID B7GSS5
Locus tag HMPREF9228_RS04920 Protein ID WP_003833460.1
Coordinates 1168506..1168829 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag HMPREF9228_RS04915 Protein ID WP_003829112.1
Coordinates 1168274..1168519 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMPREF9228_RS04895 1164113..1164775 + 663 WP_014483844.1 TetR/AcrR family transcriptional regulator -
HMPREF9228_RS04900 1164932..1166203 + 1272 WP_025301184.1 MFS transporter -
HMPREF9228_RS04905 1166387..1166578 - 192 WP_003829118.1 hypothetical protein -
HMPREF9228_RS04910 1166844..1168100 - 1257 WP_003829116.1 HipA domain-containing protein -
HMPREF9228_RS04915 1168274..1168519 + 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HMPREF9228_RS04920 1168506..1168829 + 324 WP_003833460.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HMPREF9228_RS10200 1169046..1169660 - 615 WP_014483846.1 cupin domain-containing protein -
HMPREF9228_RS04930 1169754..1170623 - 870 WP_003829104.1 class I SAM-dependent methyltransferase -
HMPREF9228_RS04935 1170861..1171226 + 366 WP_013140585.1 YccF domain-containing protein -
HMPREF9228_RS04940 1171768..1173033 + 1266 WP_014483848.1 Fic family protein -
HMPREF9228_RS10210 1173504..1173792 + 289 Protein_973 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1154625..1179060 24435


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11829.64 Da        Isoelectric Point: 6.2932

>T26375 WP_003833460.1 NC_017218:1168506-1168829 [Bifidobacterium breve ACS-071-V-Sch8b]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T26375 NC_017218:1168506-1168829 [Bifidobacterium breve ACS-071-V-Sch8b]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT26375 WP_003829112.1 NC_017218:1168274-1168519 [Bifidobacterium breve ACS-071-V-Sch8b]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT26375 NC_017218:1168274-1168519 [Bifidobacterium breve ACS-071-V-Sch8b]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0SL31

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References