Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1116550..1117186 Replicon chromosome
Accession NC_017218
Organism Bifidobacterium breve ACS-071-V-Sch8b

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag HMPREF9228_RS04675 Protein ID WP_003829234.1
Coordinates 1116830..1117186 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag HMPREF9228_RS04670 Protein ID WP_012577921.1
Coordinates 1116550..1116843 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMPREF9228_RS04645 1111964..1114642 - 2679 WP_014483804.1 alanine--tRNA ligase -
HMPREF9228_RS04650 1114738..1114974 - 237 WP_003829239.1 hypothetical protein -
HMPREF9228_RS04655 1114974..1115378 - 405 WP_014483805.1 DUF948 domain-containing protein -
HMPREF9228_RS04660 1115461..1116135 - 675 WP_015438748.1 histidine phosphatase family protein -
HMPREF9228_RS04665 1116235..1116399 + 165 Protein_915 IS30 family transposase -
HMPREF9228_RS04670 1116550..1116843 + 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HMPREF9228_RS04675 1116830..1117186 + 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HMPREF9228_RS04680 1117363..1117965 - 603 WP_003829233.1 transglutaminase family protein -
HMPREF9228_RS04685 1118070..1118426 - 357 WP_014483807.1 SdpI family protein -
HMPREF9228_RS04690 1118516..1119001 + 486 WP_014483808.1 cytidine deaminase -
HMPREF9228_RS04695 1119139..1119921 + 783 WP_014483809.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
HMPREF9228_RS04700 1120260..1120886 - 627 WP_003829226.1 30S ribosomal protein S4 -
HMPREF9228_RS04705 1121083..1121994 - 912 WP_014483811.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T26374 WP_003829234.1 NC_017218:1116830-1117186 [Bifidobacterium breve ACS-071-V-Sch8b]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T26374 NC_017218:1116830-1117186 [Bifidobacterium breve ACS-071-V-Sch8b]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT26374 WP_012577921.1 NC_017218:1116550-1116843 [Bifidobacterium breve ACS-071-V-Sch8b]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT26374 NC_017218:1116550-1116843 [Bifidobacterium breve ACS-071-V-Sch8b]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References