Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 508871..509507 Replicon chromosome
Accession NZ_CP110365
Organism Bacillus subtilis strain HY2-62

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag LFL98_RS02605 Protein ID WP_003156187.1
Coordinates 509157..509507 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag LFL98_RS02600 Protein ID WP_003225183.1
Coordinates 508871..509152 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFL98_RS02580 (LFL98_02580) 505230..505829 - 600 WP_033883543.1 rhomboid family intramembrane serine protease -
LFL98_RS02585 (LFL98_02585) 505924..506289 + 366 WP_014475874.1 holo-ACP synthase -
LFL98_RS02590 (LFL98_02590) 506455..507471 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
LFL98_RS02595 (LFL98_02595) 507586..508755 + 1170 WP_032722929.1 alanine racemase -
LFL98_RS02600 (LFL98_02600) 508871..509152 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
LFL98_RS02605 (LFL98_02605) 509157..509507 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LFL98_RS02610 (LFL98_02610) 509622..510446 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
LFL98_RS02615 (LFL98_02615) 510451..510816 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
LFL98_RS02620 (LFL98_02620) 510820..511221 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
LFL98_RS02625 (LFL98_02625) 511233..512240 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
LFL98_RS02630 (LFL98_02630) 512302..512631 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
LFL98_RS02635 (LFL98_02635) 512628..513110 + 483 WP_003234299.1 anti-sigma B factor RsbW -
LFL98_RS02640 (LFL98_02640) 513076..513864 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
LFL98_RS02645 (LFL98_02645) 513864..514463 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T263729 WP_003156187.1 NZ_CP110365:509157-509507 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT263729 WP_003225183.1 NZ_CP110365:508871-509152 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References