Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 14802..15939 Replicon plasmid unnamed1
Accession NZ_CP110361
Organism Enterococcus faecium strain AKSZ-142

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag MZO31_RS14520 Protein ID WP_024417198.1
Coordinates 15076..15939 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag MZO31_RS14515 Protein ID WP_000301765.1
Coordinates 14802..15074 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MZO31_RS14490 (MZO31_14490) 10255..10935 - 681 WP_002323195.1 IS6 family transposase -
MZO31_RS14495 (MZO31_14495) 11005..11829 + 825 Protein_17 IS1380-like element ISSsu5 family transposase -
MZO31_RS14500 (MZO31_14500) 12020..13507 + 1488 WP_077149152.1 type IA DNA topoisomerase -
MZO31_RS14505 (MZO31_14505) 13610..14506 + 897 WP_222056108.1 ParA family protein -
MZO31_RS14510 (MZO31_14510) 14570..14785 + 216 WP_001835296.1 peptide-binding protein -
MZO31_RS14515 (MZO31_14515) 14802..15074 + 273 WP_000301765.1 antitoxin Antitoxin
MZO31_RS14520 (MZO31_14520) 15076..15939 + 864 WP_024417198.1 zeta toxin family protein Toxin
MZO31_RS14525 (MZO31_14525) 16202..16939 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
MZO31_RS14530 (MZO31_14530) 17064..17147 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
MZO31_RS14535 (MZO31_14535) 17931..18182 + 252 Protein_25 hypothetical protein -
MZO31_RS14540 (MZO31_14540) 18239..18919 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
MZO31_RS14545 (MZO31_14545) 19128..19340 + 213 WP_002349227.1 hypothetical protein -
MZO31_RS14550 (MZO31_14550) 19502..20020 + 519 WP_000357244.1 YfbU family protein -
MZO31_RS14555 (MZO31_14555) 20027..20539 + 513 WP_000774078.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / poxtA / fexB / tet(L) / tet(M) - 1..54197 54197
- inside IScluster/Tn erm(B) - 10255..25800 15545


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T263713 WP_024417198.1 NZ_CP110361:15076-15939 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT263713 WP_000301765.1 NZ_CP110361:14802-15074 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References