Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 162246..163869 Replicon plasmid pECC2783_a
Accession NZ_CP110355
Organism Enterobacter hormaechei strain ECC2783

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag OLI86_RS24465 Protein ID WP_000088044.1
Coordinates 162544..163869 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag OLI86_RS24460 Protein ID WP_012477377.1
Coordinates 162246..162539 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OLI86_RS24435 (OLI86_24435) 157563..158018 + 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
OLI86_RS24440 (OLI86_24440) 158075..158437 + 363 WP_139937413.1 hypothetical protein -
OLI86_RS24445 (OLI86_24445) 159096..159752 + 657 WP_265027526.1 trypsin-like serine protease -
OLI86_RS24450 (OLI86_24450) 159955..160452 + 498 WP_000340139.1 membrane protein -
OLI86_RS24455 (OLI86_24455) 160457..161845 + 1389 WP_000083579.1 hypothetical protein -
OLI86_RS24460 (OLI86_24460) 162246..162539 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
OLI86_RS24465 (OLI86_24465) 162544..163869 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
OLI86_RS24470 (OLI86_24470) 163930..164136 + 207 WP_000134171.1 hypothetical protein -
OLI86_RS24475 (OLI86_24475) 164237..164647 + 411 WP_000985909.1 hypothetical protein -
OLI86_RS24480 (OLI86_24480) 164660..165475 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
OLI86_RS24485 (OLI86_24485) 165728..166153 + 426 WP_000278471.1 hypothetical protein -
OLI86_RS24490 (OLI86_24490) 166702..167010 + 309 WP_001224686.1 hypothetical protein -
OLI86_RS24495 (OLI86_24495) 167026..167883 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA1 / aadA5 / qacE / sul1 / armA / mph(E) / catA2 / sul2 / tet(A) / aph(6)-Id / aph(3'')-Ib / blaTEM-1B / mcr-9 - 1..286232 286232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T263708 WP_000088044.1 NZ_CP110355:162544-163869 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT263708 WP_012477377.1 NZ_CP110355:162246-162539 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References