26369

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 512342..512978 Replicon chromosome
Accession NC_017195
Organism Bacillus subtilis subsp. subtilis str. RO-NN-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag I33_RS02550 Protein ID WP_003156187.1
Coordinates 512628..512978 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag I33_RS02545 Protein ID WP_003225183.1
Coordinates 512342..512623 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I33_RS02525 508702..509301 - 600 WP_014475873.1 rhomboid family intramembrane serine protease -
I33_RS02530 509396..509761 + 366 WP_014475874.1 holo-ACP synthase -
I33_RS02535 509927..510943 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
I33_RS02540 511057..512226 + 1170 WP_014475875.1 alanine racemase -
I33_RS02545 512342..512623 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
I33_RS02550 512628..512978 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
I33_RS02555 513095..513919 + 825 WP_080009756.1 RsbT co-antagonist protein RsbRA -
I33_RS02560 513924..514289 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
I33_RS02565 514293..514694 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
I33_RS02570 514706..515713 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
I33_RS02575 515782..516111 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
I33_RS02580 516108..516590 + 483 WP_014475878.1 anti-sigma B factor RsbW -
I33_RS02585 516556..517344 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
I33_RS02590 517344..517943 + 600 WP_014475879.1 phosphoserine phosphatase RsbX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T26369 WP_003156187.1 NC_017195:512628-512978 [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T26369 NC_017195:512628-512978 [Bacillus subtilis subsp. subtilis str. RO-NN-1]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT26369 WP_003225183.1 NC_017195:512342-512623 [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT26369 NC_017195:512342-512623 [Bacillus subtilis subsp. subtilis str. RO-NN-1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References