Detailed information of TA system TA026369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 512342..512978
• Replicon: chromosome
• Accession: NC_017195
• Organism: Bacillus subtilis subsp. subtilis str. RO-NN-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: I33_RS02550
• Protein ID: WP_003156187.1
• Coordinates: 512628..512978 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: I33_RS02545
• Protein ID: WP_003225183.1
• Coordinates: 512342..512623 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I33_RS02525	508702..509301	-	600	WP_014475873.1	rhomboid family intramembrane serine protease	-
I33_RS02530	509396..509761	+	366	WP_014475874.1	holo-ACP synthase	-
I33_RS02535	509927..510943	+	1017	WP_072557167.1	outer membrane lipoprotein carrier protein LolA	-
I33_RS02540	511057..512226	+	1170	WP_014475875.1	alanine racemase	-
I33_RS02545	512342..512623	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
I33_RS02550	512628..512978	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
I33_RS02555	513095..513919	+	825	WP_080009756.1	RsbT co-antagonist protein RsbRA	-
I33_RS02560	513924..514289	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
I33_RS02565	514293..514694	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
I33_RS02570	514706..515713	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
I33_RS02575	515782..516111	+	330	WP_014475877.1	anti-sigma factor antagonist RsbV	-
I33_RS02580	516108..516590	+	483	WP_014475878.1	anti-sigma B factor RsbW	-
I33_RS02585	516556..517344	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
I33_RS02590	517344..517943	+	600	WP_014475879.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T26369 WP_003156187.1 NC_017195:512628-512978 [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T26369 NC_017195:512628-512978 [Bacillus subtilis subsp. subtilis str. RO-NN-1]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT26369 WP_003225183.1 NC_017195:512342-512623 [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT26369 NC_017195:512342-512623 [Bacillus subtilis subsp. subtilis str. RO-NN-1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32