Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 5119046..5119654 Replicon chromosome
Accession NZ_CP110346
Organism Pseudomonas aeruginosa strain PALA35

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag PALA35_RS23895 Protein ID WP_019486378.1
Coordinates 5119046..5119393 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag PALA35_RS23900 Protein ID WP_003114155.1
Coordinates 5119403..5119654 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PALA35_RS23865 (PALA35_04721) 5114405..5114677 + 273 WP_021264275.1 cysteine-rich CWC family protein -
PALA35_RS23870 (PALA35_04722) 5114677..5115369 + 693 WP_022579823.1 16S rRNA pseudouridine(516) synthase -
PALA35_RS23875 (PALA35_04723) 5115505..5116548 + 1044 WP_003134392.1 L,D-transpeptidase -
PALA35_RS23880 (PALA35_04724) 5116628..5117365 + 738 WP_003085453.1 murein L,D-transpeptidase catalytic domain family protein -
PALA35_RS23885 (PALA35_04725) 5117817..5118719 + 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
PALA35_RS23895 (PALA35_04727) 5119046..5119393 - 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PALA35_RS23900 5119403..5119654 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
PALA35_RS23905 (PALA35_04728) 5119868..5120851 - 984 WP_096225069.1 tyrosine-type recombinase/integrase -
PALA35_RS23910 (PALA35_04729) 5120851..5122143 - 1293 WP_003123045.1 hypothetical protein -
PALA35_RS23915 (PALA35_04731) 5122373..5123647 - 1275 WP_269974439.1 zonular occludens toxin family protein -
PALA35_RS23920 (PALA35_04732) 5123651..5124007 - 357 WP_269974440.1 DUF2523 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5119046..5133015 13969
- inside Prophage - - 5109962..5133015 23053


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T263650 WP_019486378.1 NZ_CP110346:c5119393-5119046 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT263650 WP_003114155.1 NZ_CP110346:c5119654-5119403 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References