Detailed information of TA system TA026362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 463771..464408
• Replicon: chromosome
• Accession: NC_017191
• Organism: Bacillus amyloliquefaciens XH7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BAXH7_RS02310
• Protein ID: WP_003156187.1
• Coordinates: 464058..464408 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BAXH7_RS02305
• Protein ID: WP_003156188.1
• Coordinates: 463771..464052 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAXH7_RS02285	460137..460736	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
BAXH7_RS02290	460829..461194	+	366	WP_013351093.1	holo-ACP synthase	-
BAXH7_RS02295	461359..462366	+	1008	WP_013351094.1	outer membrane lipoprotein carrier protein LolA	-
BAXH7_RS02300	462483..463652	+	1170	WP_013351095.1	alanine racemase	-
BAXH7_RS02305	463771..464052	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BAXH7_RS02310	464058..464408	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAXH7_RS02315	464529..465350	+	822	WP_013351096.1	STAS domain-containing protein	-
BAXH7_RS02320	465355..465720	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BAXH7_RS02325	465723..466124	+	402	WP_013351097.1	anti-sigma regulatory factor	-
BAXH7_RS02330	466136..467143	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
BAXH7_RS02335	467207..467536	+	330	WP_014469786.1	anti-sigma factor antagonist	-
BAXH7_RS02340	467533..468015	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
BAXH7_RS02345	467981..468769	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
BAXH7_RS02350	468769..469371	+	603	WP_014469787.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T26362 WP_003156187.1 NC_017191:464058-464408 [Bacillus amyloliquefaciens XH7]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T26362 NC_017191:464058-464408 [Bacillus amyloliquefaciens XH7]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT26362 WP_003156188.1 NC_017191:463771-464052 [Bacillus amyloliquefaciens XH7]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT26362 NC_017191:463771-464052 [Bacillus amyloliquefaciens XH7]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5