Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 504338..504909 Replicon chromosome
Accession NZ_CP110294
Organism Enterococcus faecium strain AKSZ-47

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag MZO30_RS03540 Protein ID WP_002286801.1
Coordinates 504568..504909 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag MZO30_RS03535 Protein ID WP_002323011.1
Coordinates 504338..504568 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MZO30_RS03510 (MZO30_03510) 499581..500912 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
MZO30_RS03515 (MZO30_03515) 500934..501560 + 627 WP_002333420.1 cysteine hydrolase -
MZO30_RS03520 (MZO30_03520) 501743..502324 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
MZO30_RS03525 (MZO30_03525) 502933..503508 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
MZO30_RS03530 (MZO30_03530) 503713..504051 - 339 WP_002286804.1 hypothetical protein -
MZO30_RS03535 (MZO30_03535) 504338..504568 + 231 WP_002323011.1 hypothetical protein Antitoxin
MZO30_RS03540 (MZO30_03540) 504568..504909 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MZO30_RS03545 (MZO30_03545) 505759..505944 + 186 WP_002304207.1 hypothetical protein -
MZO30_RS03550 (MZO30_03550) 506449..506643 + 195 WP_002295146.1 hypothetical protein -
MZO30_RS03555 (MZO30_03555) 506834..507083 + 250 Protein_480 transposase -
MZO30_RS03560 (MZO30_03560) 507327..508157 - 831 WP_265465218.1 manganese catalase family protein -
MZO30_RS03565 (MZO30_03565) 508365..509699 + 1335 WP_002333419.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T263584 WP_002286801.1 NZ_CP110294:504568-504909 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT263584 WP_002323011.1 NZ_CP110294:504338-504568 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References