Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 462474..463111 Replicon chromosome
Accession NC_017190
Organism Bacillus amyloliquefaciens LL3

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag LL3_RS02295 Protein ID WP_003156187.1
Coordinates 462761..463111 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag LL3_RS02290 Protein ID WP_003156188.1
Coordinates 462474..462755 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL3_RS02270 458840..459439 - 600 WP_013351092.1 rhomboid family intramembrane serine protease -
LL3_RS02275 459532..459897 + 366 WP_013351093.1 holo-ACP synthase -
LL3_RS02280 460062..461069 + 1008 WP_013351094.1 outer membrane lipoprotein carrier protein LolA -
LL3_RS02285 461186..462355 + 1170 WP_013351095.1 alanine racemase -
LL3_RS02290 462474..462755 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
LL3_RS02295 462761..463111 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LL3_RS02300 463232..464053 + 822 WP_013351096.1 STAS domain-containing protein -
LL3_RS02305 464058..464423 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
LL3_RS02310 464426..464827 + 402 WP_013351097.1 anti-sigma regulatory factor -
LL3_RS02315 464839..465846 + 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
LL3_RS02320 465910..466239 + 330 WP_014469786.1 anti-sigma factor antagonist -
LL3_RS02325 466236..466718 + 483 WP_013351100.1 anti-sigma B factor RsbW -
LL3_RS02330 466684..467472 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
LL3_RS02335 467472..468074 + 603 WP_014469787.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T26356 WP_003156187.1 NC_017190:462761-463111 [Bacillus amyloliquefaciens LL3]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T26356 NC_017190:462761-463111 [Bacillus amyloliquefaciens LL3]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT26356 WP_003156188.1 NC_017190:462474-462755 [Bacillus amyloliquefaciens LL3]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT26356 NC_017190:462474-462755 [Bacillus amyloliquefaciens LL3]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References