Detailed information of TA system TA026356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 462474..463111
• Replicon: chromosome
• Accession: NC_017190
• Organism: Bacillus amyloliquefaciens LL3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: LL3_RS02295
• Protein ID: WP_003156187.1
• Coordinates: 462761..463111 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: LL3_RS02290
• Protein ID: WP_003156188.1
• Coordinates: 462474..462755 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL3_RS02270	458840..459439	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
LL3_RS02275	459532..459897	+	366	WP_013351093.1	holo-ACP synthase	-
LL3_RS02280	460062..461069	+	1008	WP_013351094.1	outer membrane lipoprotein carrier protein LolA	-
LL3_RS02285	461186..462355	+	1170	WP_013351095.1	alanine racemase	-
LL3_RS02290	462474..462755	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
LL3_RS02295	462761..463111	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LL3_RS02300	463232..464053	+	822	WP_013351096.1	STAS domain-containing protein	-
LL3_RS02305	464058..464423	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
LL3_RS02310	464426..464827	+	402	WP_013351097.1	anti-sigma regulatory factor	-
LL3_RS02315	464839..465846	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
LL3_RS02320	465910..466239	+	330	WP_014469786.1	anti-sigma factor antagonist	-
LL3_RS02325	466236..466718	+	483	WP_013351100.1	anti-sigma B factor RsbW	-
LL3_RS02330	466684..467472	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
LL3_RS02335	467472..468074	+	603	WP_014469787.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T26356 WP_003156187.1 NC_017190:462761-463111 [Bacillus amyloliquefaciens LL3]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T26356 NC_017190:462761-463111 [Bacillus amyloliquefaciens LL3]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT26356 WP_003156188.1 NC_017190:462474-462755 [Bacillus amyloliquefaciens LL3]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT26356 NC_017190:462474-462755 [Bacillus amyloliquefaciens LL3]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5