26355

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 2112394..2113310 Replicon chromosome
Accession NC_017188
Organism Bacillus amyloliquefaciens TA208

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A7U5AVM4
Locus tag BAMTA208_RS10350 Protein ID WP_013351967.1
Coordinates 2112394..2113140 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag BAMTA208_RS10355 Protein ID WP_003154807.1
Coordinates 2113140..2113310 (+) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAMTA208_RS10330 2107783..2108733 - 951 WP_013351970.1 ring-cleaving dioxygenase -
BAMTA208_RS10335 2109057..2110373 + 1317 WP_013351969.1 amino acid permease -
BAMTA208_RS10340 2110658..2111275 + 618 WP_003154804.1 DUF47 domain-containing protein -
BAMTA208_RS10345 2111288..2112286 + 999 WP_013351968.1 inorganic phosphate transporter -
BAMTA208_RS10350 2112394..2113140 + 747 WP_013351967.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
BAMTA208_RS10355 2113140..2113310 + 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
BAMTA208_RS10360 2113566..2114444 - 879 WP_014470491.1 N-acetylmuramoyl-L-alanine amidase -
BAMTA208_RS10365 2114458..2114721 - 264 WP_014470492.1 phage holin -
BAMTA208_RS10370 2114735..2114998 - 264 WP_013351964.1 hemolysin XhlA family protein -
BAMTA208_RS10375 2115052..2115849 - 798 WP_014470493.1 hypothetical protein -
BAMTA208_RS10380 2115904..2116068 - 165 WP_014470494.1 XkdX family protein -
BAMTA208_RS10385 2116068..2116394 - 327 WP_014470495.1 XkdW family protein -
BAMTA208_RS10390 2116406..2118169 - 1764 WP_014470496.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2104200..2147595 43395


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-239)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29054.53 Da        Isoelectric Point: 4.6839

>T26355 WP_013351967.1 NC_017188:2112394-2113140 [Bacillus amyloliquefaciens TA208]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T26355 NC_017188:2112394-2113140 [Bacillus amyloliquefaciens TA208]
ATGCTTTTGTTTTATCAAATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTATGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGGCAATACTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGAGGGAAATATTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTTAA
CCGGAAAGACGTCTACTATGATCAAAAAGGAAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCAGTATGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT26355 WP_003154807.1 NC_017188:2113140-2113310 [Bacillus amyloliquefaciens TA208]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT26355 NC_017188:2113140-2113310 [Bacillus amyloliquefaciens TA208]
ATGGAGCAGGCGTTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCACACCGCCAGAATTTTCAAAGAAAATGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U5AVM4


Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References