Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 501708..502345 Replicon chromosome
Accession NZ_CP110268
Organism Bacillus siamensis strain YB-1631

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OM992_RS02460 Protein ID WP_003156187.1
Coordinates 501995..502345 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag OM992_RS02455 Protein ID WP_003156188.1
Coordinates 501708..501989 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM992_RS02435 (498073) 498073..498672 - 600 WP_016937174.1 rhomboid family intramembrane serine protease -
OM992_RS02440 (498765) 498765..499130 + 366 WP_095242092.1 holo-ACP synthase -
OM992_RS02445 (499295) 499295..500302 + 1008 WP_016937172.1 outer membrane lipoprotein carrier protein LolA -
OM992_RS02450 (500419) 500419..501588 + 1170 WP_016937171.1 alanine racemase -
OM992_RS02455 (501708) 501708..501989 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OM992_RS02460 (501995) 501995..502345 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OM992_RS02465 (502466) 502466..503287 + 822 WP_003156182.1 STAS domain-containing protein -
OM992_RS02470 (503292) 503292..503657 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
OM992_RS02475 (503660) 503660..504061 + 402 WP_045927020.1 anti-sigma regulatory factor -
OM992_RS02480 (504073) 504073..505080 + 1008 WP_016937170.1 PP2C family protein-serine/threonine phosphatase -
OM992_RS02485 (505144) 505144..505473 + 330 WP_016937169.1 anti-sigma factor antagonist -
OM992_RS02490 (505470) 505470..505952 + 483 WP_003156173.1 anti-sigma B factor RsbW -
OM992_RS02495 (505918) 505918..506706 + 789 WP_016937168.1 RNA polymerase sigma factor SigB -
OM992_RS02500 (506706) 506706..507308 + 603 WP_016937167.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T263543 WP_003156187.1 NZ_CP110268:501995-502345 [Bacillus siamensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT263543 WP_003156188.1 NZ_CP110268:501708-501989 [Bacillus siamensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References