Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 522236..522872 Replicon chromosome
Accession NZ_CP110264
Organism Bacillus halotolerans strain HMB20199

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OMK57_RS02670 Protein ID WP_003156187.1
Coordinates 522522..522872 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag OMK57_RS02665 Protein ID WP_003225183.1
Coordinates 522236..522517 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OMK57_RS02645 (OMK57_02645) 518593..519192 - 600 WP_264798263.1 rhomboid family intramembrane serine protease -
OMK57_RS02650 (OMK57_02650) 519287..519652 + 366 WP_069487530.1 holo-ACP synthase -
OMK57_RS02655 (OMK57_02655) 519818..520834 + 1017 WP_082687822.1 outer membrane lipoprotein carrier protein LolA -
OMK57_RS02660 (OMK57_02660) 520951..522120 + 1170 WP_044161733.1 alanine racemase -
OMK57_RS02665 (OMK57_02665) 522236..522517 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OMK57_RS02670 (OMK57_02670) 522522..522872 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OMK57_RS02675 (OMK57_02675) 522990..523814 + 825 WP_082687807.1 RsbT co-antagonist protein RsbRA -
OMK57_RS02680 (OMK57_02680) 523819..524184 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
OMK57_RS02685 (OMK57_02685) 524188..524589 + 402 WP_024714447.1 serine/threonine-protein kinase RsbT -
OMK57_RS02690 (OMK57_02690) 524601..525608 + 1008 WP_044161721.1 phosphoserine phosphatase RsbU -
OMK57_RS02695 (OMK57_02695) 525669..525998 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
OMK57_RS02700 (OMK57_02700) 525995..526477 + 483 WP_024120302.1 anti-sigma B factor RsbW -
OMK57_RS02705 (OMK57_02705) 526443..527231 + 789 WP_024120303.1 RNA polymerase sigma factor SigB -
OMK57_RS02710 (OMK57_02710) 527231..527830 + 600 WP_044161719.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T263523 WP_003156187.1 NZ_CP110264:522522-522872 [Bacillus halotolerans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT263523 WP_003225183.1 NZ_CP110264:522236-522517 [Bacillus halotolerans]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References