Detailed information of TA system TA026350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 457741..458378
• Replicon: chromosome
• Accession: NC_017188
• Organism: Bacillus amyloliquefaciens TA208

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BAMTA208_RS02275
• Protein ID: WP_003156187.1
• Coordinates: 458028..458378 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BAMTA208_RS02270
• Protein ID: WP_003156188.1
• Coordinates: 457741..458022 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMTA208_RS02250	454107..454706	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
BAMTA208_RS02255	454799..455164	+	366	WP_013351093.1	holo-ACP synthase	-
BAMTA208_RS02260	455329..456336	+	1008	WP_013351094.1	outer membrane lipoprotein carrier protein LolA	-
BAMTA208_RS02265	456453..457622	+	1170	WP_013351095.1	alanine racemase	-
BAMTA208_RS02270	457741..458022	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BAMTA208_RS02275	458028..458378	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAMTA208_RS02280	458499..459320	+	822	WP_013351096.1	STAS domain-containing protein	-
BAMTA208_RS02285	459325..459690	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BAMTA208_RS02290	459693..460094	+	402	WP_013351097.1	anti-sigma regulatory factor	-
BAMTA208_RS02295	460106..461113	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
BAMTA208_RS02300	461177..461506	+	330	WP_014469786.1	anti-sigma factor antagonist	-
BAMTA208_RS02305	461503..461985	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
BAMTA208_RS02310	461951..462739	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
BAMTA208_RS02315	462739..463341	+	603	WP_014469787.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T26350 WP_003156187.1 NC_017188:458028-458378 [Bacillus amyloliquefaciens TA208]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T26350 NC_017188:458028-458378 [Bacillus amyloliquefaciens TA208]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT26350 WP_003156188.1 NC_017188:457741-458022 [Bacillus amyloliquefaciens TA208]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT26350 NC_017188:457741-458022 [Bacillus amyloliquefaciens TA208]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5