Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2814441..2815092 Replicon chromosome
Accession NZ_CP110214
Organism Lacticaseibacillus paracasei strain ATG-E1

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag OKX02_RS13755 Protein ID WP_003567661.1
Coordinates 2814441..2814824 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag OKX02_RS13760 Protein ID WP_003567665.1
Coordinates 2814844..2815092 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OKX02_RS13750 (OKX02_13750) 2813736..2814257 - 522 WP_003567657.1 QueT transporter family protein -
OKX02_RS13755 (OKX02_13755) 2814441..2814824 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OKX02_RS13760 (OKX02_13760) 2814844..2815092 - 249 WP_003567665.1 antitoxin Antitoxin
OKX02_RS13765 (OKX02_13765) 2815160..2816296 - 1137 WP_003581082.1 alanine racemase -
OKX02_RS13770 (OKX02_13770) 2816283..2816657 - 375 WP_003567669.1 holo-ACP synthase -
OKX02_RS13775 (OKX02_13775) 2816826..2818334 - 1509 WP_265013270.1 DEAD/DEAH box helicase -
OKX02_RS13780 (OKX02_13780) 2818460..2818603 - 144 WP_016380391.1 hypothetical protein -
OKX02_RS13785 (OKX02_13785) 2818634..2820022 - 1389 WP_003581096.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T263430 WP_003567661.1 NZ_CP110214:c2814824-2814441 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT263430 WP_003567665.1 NZ_CP110214:c2815092-2814844 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References