Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4597381..4598191 Replicon chromosome
Accession NZ_CP110170
Organism Klebsiella pneumoniae strain YZ22PK089

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag OK017_RS22700 Protein ID WP_004178461.1
Coordinates 4597381..4597914 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OK017_RS22705 Protein ID WP_002887278.1
Coordinates 4597925..4598191 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OK017_RS22695 (4596212) 4596212..4597333 + 1122 WP_004186850.1 cupin domain-containing protein -
OK017_RS22700 (4597381) 4597381..4597914 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
OK017_RS22705 (4597925) 4597925..4598191 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OK017_RS22710 (4598294) 4598294..4599727 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
OK017_RS22715 (4599717) 4599717..4600400 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
OK017_RS22720 (4600573) 4600573..4601958 + 1386 WP_064152126.1 efflux transporter outer membrane subunit -
OK017_RS22725 (4601976) 4601976..4602320 + 345 WP_064152125.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T263339 WP_004178461.1 NZ_CP110170:c4597914-4597381 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT263339 WP_002887278.1 NZ_CP110170:c4598191-4597925 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References