Detailed information of TA system TA263272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 18764..19674
• Replicon: plasmid unnamed1
• Accession: NZ_CP110151
• Organism: Klebsiella pneumoniae strain YZ22CS072

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: OK018_RS25425
• Protein ID: WP_032413375.1
• Coordinates: 19204..19674 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: OK018_RS25420
• Protein ID: WP_032413443.1
• Coordinates: 18764..19207 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OK018_RS25385	14042..15580	-	1539	WP_064824457.1	IS66-like element ISKpn24 family transposase	-
OK018_RS25390	15629..15976	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
OK018_RS25395	15973..16359	-	387	WP_127114079.1	transposase	-
OK018_RS25400	16421..17389	-	969	WP_164973615.1	IS5-like element IS903B family transposase	-
OK018_RS25405	17421..17846	-	426	Protein_18	transposase	-
OK018_RS25410	17866..18051	+	186	WP_071571073.1	DUF2188 domain-containing protein	-
OK018_RS25415	18152..18664	+	513	Protein_20	DUF4113 domain-containing protein	-
OK018_RS25420	18764..19207	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
OK018_RS25425	19204..19674	+	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
OK018_RS25430	19784..20044	-	261	WP_017901409.1	hypothetical protein	-
OK018_RS25435	20616..20993	-	378	Protein_24	integrase core domain-containing protein	-
OK018_RS25440	21067..21260	+	194	Protein_25	transposase	-
OK018_RS25445	21383..22333	+	951	WP_077268727.1	IS5-like element IS903B family transposase	-
OK018_RS25450	22344..22871	+	528	WP_224707344.1	hypothetical protein	-
OK018_RS25455	22812..22967	-	156	Protein_28	transposase	-
OK018_RS25460	23703..23795	-	93	Protein_29	hypothetical protein	-
OK018_RS25465	23837..24373	+	537	WP_032437437.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / blaTEM-1B / aac(3)-IId / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(6)-Id / aph(3'')-Ib / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4	-	1..224600	224600
	-	inside	IScluster/Tn	-	-	14042..26783	12741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T263272 WP_032413375.1 NZ_CP110151:19204-19674 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT263272 WP_032413443.1 NZ_CP110151:18764-19207 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4