Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4918..5654 Replicon plasmid pMX581-77k
Accession NZ_CP110125
Organism Klebsiella michiganensis strain MX581

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag OKE89_RS28750 Protein ID WP_003026803.1
Coordinates 5172..5654 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag OKE89_RS28745 Protein ID WP_003026799.1
Coordinates 4918..5184 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OKE89_RS28720 (OKE89_28715) 1..1011 + 1011 WP_000200070.1 RepB family plasmid replication initiator protein -
OKE89_RS28725 (OKE89_28720) 1709..2449 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
OKE89_RS28730 (OKE89_28725) 2813..3781 - 969 WP_023307223.1 IS5-like element IS903B family transposase -
OKE89_RS28735 (OKE89_28730) 4026..4229 + 204 WP_004213596.1 HHA domain-containing protein -
OKE89_RS28740 (OKE89_28735) 4243..4473 + 231 WP_023307222.1 hypothetical protein -
OKE89_RS28745 (OKE89_28740) 4918..5184 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
OKE89_RS28750 (OKE89_28745) 5172..5654 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
OKE89_RS28755 (OKE89_28750) 5855..7258 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
OKE89_RS28760 (OKE89_28755) 7287..7919 - 633 WP_001567369.1 hypothetical protein -
OKE89_RS28765 (OKE89_28760) 8149..9492 + 1344 WP_080339306.1 ISNCY family transposase -
OKE89_RS28770 (OKE89_28765) 9563..10381 - 819 WP_023307220.1 abortive infection family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..77402 77402
- inside IScluster/Tn - - 2813..32871 30058


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T263169 WP_003026803.1 NZ_CP110125:5172-5654 [Klebsiella michiganensis]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT263169 WP_003026799.1 NZ_CP110125:4918-5184 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References