Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2068682..2069272 Replicon chromosome
Accession NZ_CP110123
Organism Klebsiella michiganensis strain MX581

Toxin (Protein)


Gene name pemK Uniprot ID A0A7H5AAK0
Locus tag OKE89_RS09945 Protein ID WP_004852304.1
Coordinates 2068940..2069272 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag OKE89_RS09940 Protein ID WP_004852307.1
Coordinates 2068682..2068939 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OKE89_RS09920 (OKE89_09920) 2064580..2065185 - 606 WP_049106037.1 glutathione S-transferase family protein -
OKE89_RS09925 (OKE89_09925) 2065365..2066276 + 912 WP_049106038.1 LysR substrate-binding domain-containing protein -
OKE89_RS09930 (OKE89_09930) 2066329..2067327 - 999 WP_264607073.1 aldo/keto reductase -
OKE89_RS09935 (OKE89_09935) 2067428..2068342 + 915 WP_004852309.1 LysR family transcriptional regulator -
OKE89_RS09940 (OKE89_09940) 2068682..2068939 + 258 WP_004852307.1 antitoxin Antitoxin
OKE89_RS09945 (OKE89_09945) 2068940..2069272 + 333 WP_004852304.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OKE89_RS09950 (OKE89_09950) 2069591..2070952 - 1362 WP_003036316.1 HEPN/Toprim-associated domain-containing protein -
OKE89_RS09955 (OKE89_09955) 2071788..2072726 + 939 WP_003036321.1 hypothetical protein -
OKE89_RS09960 (OKE89_09960) 2072802..2073284 + 483 WP_003036324.1 hypothetical protein -
OKE89_RS09965 (OKE89_09965) 2073306..2074109 + 804 WP_003036327.1 TIGR02391 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11863.81 Da        Isoelectric Point: 10.5834

>T263161 WP_004852304.1 NZ_CP110123:2068940-2069272 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGIKTLGVIRCDQPRTI
DMTARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT263161 WP_004852307.1 NZ_CP110123:2068682-2068939 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H5AAK0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References