Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1462480..1463114 Replicon chromosome
Accession NZ_CP110123
Organism Klebsiella michiganensis strain MX581

Toxin (Protein)


Gene name vapC Uniprot ID A0A7H5A909
Locus tag OKE89_RS07110 Protein ID WP_004853162.1
Coordinates 1462710..1463114 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2J4P7G5
Locus tag OKE89_RS07105 Protein ID WP_004853165.1
Coordinates 1462480..1462710 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OKE89_RS07090 (OKE89_07090) 1457594..1458847 + 1254 WP_004853170.1 site-specific integrase -
OKE89_RS07095 (OKE89_07095) 1458900..1460558 + 1659 WP_264605001.1 hypothetical protein -
OKE89_RS07100 (OKE89_07100) 1460624..1461499 - 876 WP_004853166.1 integrase domain-containing protein -
OKE89_RS07105 (OKE89_07105) 1462480..1462710 + 231 WP_004853165.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
OKE89_RS07110 (OKE89_07110) 1462710..1463114 + 405 WP_004853162.1 tRNA(fMet)-specific endonuclease VapC Toxin
OKE89_RS07115 (OKE89_07115) 1463219..1464232 - 1014 WP_004853161.1 Gfo/Idh/MocA family oxidoreductase -
OKE89_RS07120 (OKE89_07120) 1464243..1465223 - 981 WP_004853159.1 PTS glucitol/sorbitol transporter subunit IIB -
OKE89_RS07125 (OKE89_07125) 1465220..1465594 - 375 WP_004853156.1 PTS glucitol/sorbitol transporter subunit IIA -
OKE89_RS07130 (OKE89_07130) 1465591..1466112 - 522 WP_004853154.1 PTS glucitol/sorbitol transporter subunit IIC -
OKE89_RS07135 (OKE89_07135) 1466476..1466949 + 474 WP_032721562.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1449418..1478822 29404


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15005.36 Da        Isoelectric Point: 7.8441

>T263160 WP_004853162.1 NZ_CP110123:1462710-1463114 [Klebsiella michiganensis]
MLKYMLDTNICIYTIKNKPQAVREAFNQHYGRMCISSVTLMELIYGAEKSASPEKNLRVVEGFIARLEVLNYGIDVAVQT
GQIRAELARAGTPVGPYDSMIAAHARALGLILVTNNTREFERINGLRLEDWSIS

Download         Length: 405 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8465.44 Da        Isoelectric Point: 4.7527

>AT263160 WP_004853165.1 NZ_CP110123:1462480-1462710 [Klebsiella michiganensis]
MEKTTVFKSNRSQAVRLPKAVALPEDVKHVDIVAVGRTRIITPAGESWDSWFDGESVTSDFMAAREQPGDQDREGF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H5A909

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4P7G5

References